吸入细颗粒物对肺损伤以及肉肠道微生物群的影响
Ying Zhou1, Bin Xu1, Linyi Wang1
1Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Poultry science
|February 9, 2024
概括
细颗粒物 (PM2.5) 暴露会引发炎症并改变肠道微生物群,从而导致 brojlers 的肺损伤. 这种肠道失调会增加肠道衍生的脂多糖,可能会恶化肺损伤.
科学领域:
- 环境健康 环境健康
- 微生物学 微生物学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 细颗粒物 (PM2.5) 是影响动物和人类健康,特别是呼吸系统健康的环境风险因素.
- 肠道微生物群在肺损伤中发挥作用,但其对暴露于PM2.5的肉肠肺轴的具体影响尚不清楚.
研究的目的:
- 为了研究PM2.5暴露对肉肠肺轴的影响.
- 在肉模型中探索PM2.5诱导的肺损伤的潜在机制.
主要方法:
- 建立了一个使用3个星期老的雄性Arbor Acres肉的肉模型.
- 分析包括肺炎标志物 (IL-6,IFN-γ,TNF-α,IL-10),肠道微生物群多样性 (α和β多样性),特定细菌丰富性,肠道屏障完整性 (muc2,claudin-1) 和脂多糖 (LPS) 水平.
- 斯皮尔曼相关性分析被用来评估肠道微生物群和肺炎之间的关系.
主要成果:
- 暴露于PM2.5触发了肺部的TLR4信号通路,增加了促炎细胞因子 (IL-6,IFN-γ,TNF-α) 和降低了IL-10.
- 肠道微生物群的多样性发生了显著的变化,α多样性减少,β多样性改变. 特定细菌的丰富性发生了变化,增加了Escherichia-Shigella,减少了DTU089,Ocillospirales和Staphylococcus.
- 肠上皮质屏障功能被破坏,增加了肠道透性和LPS的转位,这与肺炎标志物 (TLR4,TNF-α,IFN-γ) 有积极的相关性.
结论:
- 暴露于PM2.5会通过炎症和TLR4通路激活诱导 brojlers的肺损伤.
- 暴露于PM2.5会导致肠道微生物群失生,导致肠道衍生的LPS增加,并可能导致肺损伤.
- 肠道微生物群失调与PM2.5诱导的肺损伤有关,这表明它是减轻环境污染物影响的潜在监管目标.
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