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太平洋白Litopenaeus vannamei中SRC基因的结构和功能分析

Shuqing Si¹, Xiaojun Zhang¹, Yang Yu²

¹CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Chinese Academy of Sciences, Wuhan, 430072, China; College of Earth Science, University of Chinese Academy of Sciences, Beijing, 100049, China.

Fish & shellfish immunology

|February 9, 2024

概括

中的食尸受体C类 (SRC) 基因影响身体体重和生长. 抑制LvSRC减少了的大小,影响了生长和免疫路径,为水产养殖提供了洞察力.

科学领域:

水产养殖遗传学水产养殖遗传学
无脊椎动物动物学
分子生物学分子生物学

背景情况:

在无脊椎动物中发现了Scavenger受体C类 (SRC) 基因.
以前的研究将SRC与太平洋白 (Litopenaeus vannamei) 的体重联系起来.
对于LvSRC在生长中的作用的具体机制需要阐明.

研究的目的:

为了研究Litopenaeus vannamei.LvSRC基因的结构,系系,表达和功能.
了解LvSRC影响生长和发育的分子机制.
探索LvSRC在遗传学和繁殖中的潜在应用.

主要方法:

对LvSRC基因的结构和遗传学分析.
基因表达在不同组织和生长率群体中的概况.
通过RNA干扰 (RNAi) 来研究LvSRC的功能.
RNA测序 (RNA-seq) 用于识别差异表达基因 (DEG).
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.

主要成果:

LvSRC拥有两个CCP域和一个MAM域,在心脏中表达最高.
在快速生长的中观察到更高的LvSRC表达.
通过RNAi介导的LvSRC的淘汰显著抑制了身体长度和体重增加.
通过RNA-seq检测,发现了598种参与生长,免疫和新陈代谢的DEG.
GO和KEGG分析显示,生长,脂质代谢和免疫反应的途径发生了显著的改变.

结论:

在Litopenaeus vannamei中,LvSRC在调节体重和生长方面发挥着至关重要的作用.
LvSRC参与了各种生理过程,包括免疫调节和脂质代谢.
了解LvSRC的功能为养殖中的遗传改进和繁殖策略提供了基础.

关键词:

基因表达基因表达基因表达基因结构和基因功能增长的增长增长的增长利托佩内亚斯 (Litopenaeus vannamei) 是一个水族群.在SRC中,它是SRC.

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