凝血因子X激活的动力学和调节通过脂膜上的内在张力作用激活凝血因子X
Anastasia N Sveshnikova1, Alexey M Shibeko2, Tatiana A Kovalenko2
1National Medical and Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, 1 Samory Mashela St, Moscow, 117198, Russia; Faculty of Fundamental Physico-Chemical Engineering, Lomonosov Moscow State University, 1/51 Leninskie Gory, 119991 Moscow, Russia; Department of Normal Physiology, Sechenov First Moscow State Medical University, 8/2 Trubetskaya St., 119991 Moscow, Russia; Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 4 Kosygina St, Moscow, 119991, Russia.
内在的张力酶复合体.
科学领域:
- 生物化学 生物化学
- 计算生物学 计算生物学
- 血液学 血液学 血液学
背景情况:
- 血液凝固依赖于与脂结合的内在张力复合体来激活X因子.
- 内部张力调节机制和基质传递通路的理解仍然不充分.
- 有关在位复杂组装中的扩散限制存在开放的问题.
研究的目的:
- 开发和分析脂表面内在张力的详细计算机模型.
- 为了研究内在张力复合体形成和基质输送的动态.
- 阐明扩散和流动在X因子激活中的作用.
主要方法:
- 利用一种机械驱动的计算机模型来实现内在的张力.
- 采用了三维反应-扩散-宣传模拟.
- 嵌入的随机模拟,如果适合系统分析.
主要成果:
- 在生理条件下,系统动态主要是非静止的.
- 需要依赖膜和依赖溶液的基质输送途径来描述数据.
- 第八个因子a-X因子复合物的形成是主要的组装途径,显示出高脂质依赖的亲和力.
- 扩散限制对XA因子产生的影响很小.
- 通过增强辅因子供应,流量加速了XA因子的产生.
结论:
- 内在张力作为一个非静止系统运行.
- 使用多种基质输送途径,根据条件而有所不同.
- 基质的扩散并不是XA因子生产的重大限制.
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