从下生物膜中分离出来的Schaalia odontolytica的完整基因组序列
Eun-Young Jang1,2, Jeewan Chun1,2, Kyu Hwan Kwack1
1Department of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul, 02447, Republic of Korea.
BMC genomic data
|February 9, 2024
概括
从牙周炎中分离出来的Schaalia odontolytica菌株 KHUD_008 的基因组测序揭示了亚种级的遗传变异. 对比分析确定了与细胞外和DNA修复相关的菌株特异性基因,增强了我们对这种口腔细菌的理解.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 口腔健康 口腔健康
背景情况:
- 最近的分类学重新分类为某些Actinomyces物种提出了新的属,包括Schaalia.
- 这种新属中的一种物种Schaalia odontolytica,通常在人类口腔中发现,并与菌病变有关.
- 对于S. odontolytica,基因组数据有限,特别是在亚种水平的变化方面.
研究的目的:
- 为了执行Schaalia odontolytica菌株 KHUD_008的全基因组测序,该菌株是从一名韩国牙周炎患者中分离出来的.
- 进行对 KHUD_008 菌株与现有的 S. odontolytica 基因组 (XH001 和 FDAARGOS_732) 的比较基因组分析.
- 调查S. odontolytica. 中的亚种级遗传多样性.
主要方法:
- 使用太平洋生物科学第二季测序数据和微生物基因组分析管道的新基因组组.
- 基因组完整性的评估使用基准测试通用单拷贝正义词 (BUSCO),达到99.2%的完整性.
- 对比基因组分析以确定菌株特定的基因和变异.
主要成果:
- 组装的S. odontolytica KHUD_008的基因组为2,389,595个基因组,GC含量为66.37%,含有2,002个蛋白质编码基因,9个rRNA和48个tRNA.
- 对比分析显示,与XH001和FDAARGOS_732.8菌株相比,KHUD_008菌株中有许多菌株特异性基因.
- 这些独特的基因主要与包膜生物发生和复制/重组/修复途径有关.
结论:
- 基因组测序和比较分析为Schaalia odontolytica的遗传多样性提供了宝贵的见解.
- 鉴定出的菌株特异性基因可能有助于口腔环境中不同S. odontolytica菌株的适应性和致病性.
- 这项研究扩大了S. odontolytica的基因组资源,促进了未来对其在口腔健康和疾病中的作用的研究.
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