相关实验视频

Updated: Jun 21, 2026

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

芯片上的血管化心脏球体,用于测试治疗药物的毒性

Stefania Di Cio^1,2, Emilie Marhuenda^1,2, Malcolm Haddrick³

¹Institute of Bioengineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK.

Scientific reports

|February 9, 2024

概括

研究人员在微流体芯片中开发了微血管化的心脏球体,用于药物测试. 这些模型模仿体内条件,显示稳定的血管网络和持续的心肌细胞功能超过25天.

科学领域:

生物医学工程生物医学工程
心血管研究研究心血管研究
药物发现药物发现药物发现

背景情况:

器官芯片技术正在推进治疗测试.
为药物运输研究重建微血管化组织模型仍然具有挑战性.
模拟全身药物输送需要采用能够捕捉跨内皮细胞运输和组织吸收的模型.

研究的目的:

在微流体芯片中开发和描述微血管化的心脏球体.
评估心脏球状体内的血管网络的整合和稳定性.
评估开发模型的功能性能和治疗测试能力.

主要方法:

制造具有多间隔设计的微流体芯片.
嵌入心脏球状体和与皮细胞共同培养.
评估血管网络的形成,标记物表达 (心脏和内皮) 和系统 perfusion.
长期监测心肌细胞跳动和功能表现.
概念验证的治疗毒性测试.

主要成果:

成功形成稳定的微血管网络,与心脏球形相结合.
持续表达心肌细胞标记物 (例如cTNT) 和功能性细胞骨架网络.
持续的心肌细胞跳动超过25天,证明了透气性和功能性能的表现.
悬浮球体和血管化模型之间的治疗毒性差异的识别.

结论:

嵌入微血管化心脏球体的微流体芯片为研究药物运输和疗效提供了一个强大的平台.
该模型准确地复制了心脏组织生理学的关键方面,包括血管化和收缩性.
与传统方法相比,这种先进的体外模型为治疗安全性和疗效测试提供了更好的预测能力.

更多相关视频

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Published on: January 23, 2021

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Published on: March 10, 2023

相关概念视频

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...

Dysrhythmias V: Evaluating Dysrhythmias

Dysrhythmias V: Evaluating Dysrhythmias

Dysrhythmias, also known as arrhythmias, are disturbances in the heart's rhythm that range from benign to life-threatening. A thorough evaluation is crucial for appropriate management and involves a comprehensive medical history, physical examination, and various diagnostic tests.Medical HistorySymptoms: Collect detailed information on palpitations, dizziness, syncope, chest pain, and fatigue. Note their onset, frequency, and triggers.Previous Cardiac Issues: Document any history of heart...