相关实验视频
Updated: Jul 4, 2025

06:31
Step-specific Sorting of Mouse Spermatids by Flow Cytometry
Published on: December 31, 2015
10.6K
在Sargassum horneri的高度凝结的精子核中,精子特异性基因素H1
Yu Takeuchi1, Shinya Sato1, Chikako Nagasato2
1Faculty of Marine Science and Technology, Fukui Prefectural University, 1-1 Gakuencho, Obama, Fukui, 917-0003, Japan.
Scientific reports
|February 9, 2024
概括
这项研究确定了棕藻精子中的基因组H1蛋白,揭示了ShH1.4作为潜在的精子特异基因组,参与精子生成期间的染色质凝聚.
科学领域:
- * * 海洋生物学 海洋生物学
- * 分子生物学 * 分子生物学
- * 生物化学 * 生物化学
背景情况:
- * 精子生成涉及各种生物体的精子核中显著的染色质凝聚.
- *精子核基本蛋白 (SNBPs) 对于这种凝结至关重要,但它们在棕藻 (Fucales) 中的特定作用在很大程度上仍未被描述.
- * 富卡莱斯系,包括棕色藻类如 Sargassum horneri,表现出带有凝聚核的精子,表明存在特定的SNBPs.
研究的目的:
- * 为了识别和描述棕色藻类Sargassum horneri.中的精子核基本蛋白 (SNBPs).
- * 为了研究在S. horneri的精子生成过程中,组织基因H1基因的基因表达模式.
- * 为了确定任何已识别的基因组H1蛋白是否在这种物种中充当精子特异性的SNBPs.
主要方法:
- *转录组数据分析,以识别S. horneri.中的基因组H1基因.
- *二甲基硫酸盐-多烯胺凝电泳 (SDS-PAGE) 用于蛋白质分离.
- * 液体染色学-双重质谱法 (LC-MS/MS) 用于蛋白质的鉴定.
- *使用转录基因数据进行定量基因表达分析.
主要成果:
- * 在S. horneri.中发现了6个组素H1基因 (ShH1.1a,ShH1b,ShH1.2,ShH1.3,ShH1.4,ShH1.5).
- * SDS-PAGE和LC-MS/MS证实精子核中存在核心质子和SH1.2,SH1.3和SH1.4的存在.
- *ShH1.2和ShH1.3基因在植物和生殖组织中表达,而ShH1.4在男性生殖器官中特别表达.
结论:
- * Sargassum horneri的精子核含有特定的素H1蛋白质,包括ShH1.2,ShH1.3和ShH1.4.
- *ShH1.4的基因表达模式表明它是S. horneri.中的精子特异性基因素H1.
- *这些发现有助于理解棕藻在精子生成过程中染色质凝聚的分子机制.
相关概念视频
Spermatogenesis
102.5K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.5K
Sperm Structure and Semen Composition
2.1K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
2.1K
Histone Variants at the Centromere
4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
Fertilization
71.0K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
71.0K
Heterochromatin
12.7K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
12.7K
Sperm Transport
820
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
820

