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基于TALE的有机细胞基因组编辑和植物基因表达

Jer-Young Lin^1,2, Yu-Chang Liu¹, Yan-Hao Tseng²

¹Agricultural Biotechnology Research Center, Academia Sinica, Tainan, 71150, Taiwan.

Plant cell reports

|February 9, 2024

概括

基于转录激活器类效应器 (TALE) 的编辑器为工程植物器官基因组提供了一种新方法. 本综述详细介绍了基于TALE的器官细胞基因组编辑的最新进展,克服了其他技术的局限性.

科学领域:

植物分子生物学植物分子生物学
遗传学是一个遗传学.
生物技术是生物技术.

背景情况:

基因编辑对于植物研究和生物技术至关重要.
克里斯普尔/Cas9广泛用于核基因组编辑,但在植物有机体中面临着交付挑战.
传统的 хлоропласт转化是物种有限的,在技术上具有挑战性.

研究的目的:

审查基于TALE的植物有机细胞基因组编辑的最新进展.
突出基于TALE的编辑器作为对有机体基因组工程现有方法的替代方案.
讨论TALE技术在植物有机体修饰方面的优势.

主要方法:

对基于TALE的植物器官基因组编辑现有文献的综述.
讨论TALE交付的Agrobacteria介导的核转化.
对TALE编辑器向和植物有机体表达的分析.

主要成果:

基于TALE的编辑器可以有效地交付和表达在植物有机体中.
塔尔技术绕过了与CRISPR/Cas9传递到器官相关的限制.
基于TALE的编辑为工程质体和潜在的线粒体基因组提供了可行的替代方案.

结论:

基于TALE的编辑器为工程植物器官基因组提供了一个有希望的替代方案.
这项技术克服了叶绿体转化和植物中缺乏直接线粒体转化方法的挑战.
最近的发展表明,基于TALE的器官细胞基因组编辑应用程序取得了显著进展.

关键词:

塑是一种塑.基因编辑基因编辑线粒体中的线粒体.器官细胞基因组编辑 "一个故事"的故事.

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