使用体外发酵模型对性结肠炎中益生菌候选物的比较
James M Kennedy1,2, Aminda De Silva2, Gemma E Walton1
1Department of Food and Nutritional Sciences, The University of Reading, Reading RG6 6AP, United Kingdom.
Journal of applied microbiology
|February 10, 2024
概括
在性结肠炎患者中,三种益生菌,包括2'-fucosyllactose (2'-FL),显著改善了肠道微生物群和增加了短链脂肪酸 (SCFA). 2'-FL显著增强了酸盐和抑制了一个关键的病原体.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 营养科学 营养科学
背景情况:
- 性结肠炎 (UC) 是一种具有改变肠道微生物群的慢性炎症性肠病.
- 短链脂肪酸 (SCFA) 在肠道健康和炎症调节中起着至关重要的作用.
- 益生菌是不可消化的食品成分,通过调节肠道微生物群,对宿主产生有益影响.
研究的目的:
- 研究三种益生菌对UC患者便微生物群的体外影响.
- 为了比较2 -fucosyllactose (2 -FL),果糖-寡糖化物 (FOS) 和银糖-寡糖化物 (GOS) 对细菌群体和SCFA生产的影响.
- 通过调节肠道环境来评估这些益生菌在管理UC方面的潜力.
主要方法:
- 在实验室中使用了pH控制的批量培养发酵模型.
- 来自健康对照组和UC患者的便样本在48小时内用益生菌发酵.
- 进行了细菌计数 (光 in situ 杂交,流细胞计) 和SCFA量化 (气色谱-质谱).
主要成果:
- 所有测试的益生菌 (2-FL,FOS,GOS) 都积极调节了肠道微生物群,并增加了SCFA总度.
- 2 -FL显著增加了酸盐的产量,并产生了最高的SCFA总度.
- 2 -FL表现出最强的抑制Desulfovibrio spp.,一个UC相关的病原体.
结论:
- 2 -FL,FOS和GOS显示出改善UC患者肠道微生物群组成的显著潜力.
- 益生菌补充剂可以增强SCFA的产生,这是肠道健康的关键因素.
- 2 -FL因其广泛的有益作用而成为UC管理的有希望的益生菌.
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