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影响唾液中细胞外DNA的分析前因素

Ľubica Janovičová¹, Dominika Holániová¹, Barbora Vlková¹

¹Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, 811 08 Bratislava, Slovakia.

Diagnostics (Basel, Switzerland)

|February 10, 2024

概括

唾液细胞外DNA (ecDNA) 是一种潜在的疾病生物标志物. 额外的离心法显著降低了唾液中的ecDNA度,而冷的影响很小,这表明ecDNA与细胞碎片有关.

科学领域:

分子生物学分子生物学
生物化学生物化学
生物标志物发现发现

背景情况:

唾液DNA很容易收集用于遗传分析.
唾液中的细胞外DNA (ecDNA) 显示出作为口腔和全身疾病生物标志物的潜力.
分析前样本处理会影响唾液中的ecDNA数量,但影响尚不清楚.

研究的目的:

为了研究离心和冷对唾液ecDNA度的影响.
为了确定最佳的分析前处理,以保存唾液ecDNA.

主要方法:

从15名健康志愿者收集的非刺激的唾液样本.
样品经过初始离心 (1600 x g) 并冷或加工新鲜.
在新鲜和解的样本上进行了第二次高速离心 (16,000 x g).
从超级生物中分离出DNA,并使用度测量和实时PCR (准核和线粒体DNA) 来量化.

主要成果:

结对唾液中ecDNA度的影响微不足道.
在新鲜和冷样本中,额外的离心步骤显著减少了超过97%的ecDNA.
定量PCR证实核和线粒体ecDNA序列在离心后显著下降.

结论:

唾液ecDNA度主要受到额外离心的影响,而不是样品的冷.
这些发现表明,唾液ecDNA在很大程度上与细胞残骸和亡体有关.
未来的研究应该专注于特定疾病的唾液ecDNA部分.

关键词:

没有细胞的DNA DNA.这些微粒是微粒.中性粒细胞外细胞陷牙周炎是指牙周炎的一种疾病.唾液唾液唾液是一种

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