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防务监管网络与大米中的circRNA相关,以应对棕色虫侵袭

Hou-Hong Yang¹, Ya-Xuan Wang¹, Jing Xiao¹

¹State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.

Plants (Basel, Switzerland)

|February 10, 2024

概括

循环RNAs (circRNAs) 参与了对棕色植物 (BPH) 的防护. 这项研究确定了差异表达的circRNA及其点,揭示了它们在植物昆虫相互作用中的作用.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
昆虫学昆虫学是一门学科.

背景情况:

棕色虫 (BPH) 是一种主要的水害虫,现有的抵抗机制由于毒性种群而失败.
循环RNAs (circRNAs) 涉及植物与环境的相互作用,但它们在昆虫抵抗中的作用尚不清楚.

研究的目的:

为了对米环RNAs进行全基因组分析,以应对BPH感染.
为了阐明大米中circRNA介导的昆虫耐药性的机制.

主要方法:

使用高通量测序,在不同BPH毒性组 (IR-IR56-BPH,IR-TN1-BPH) 和对照组 (IR-CK) 中识别 (IR56) 中的circRNA.
进行了差异表达分析,基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 丰富分析.
评估了精选的circRNA的验证,以及它们对微RNA (miRNA) 和信使RNA (mRNA) 的调控影响.

主要成果:

总共有186个circRNAs被确定,在BPH感染的反应中,有相当数量的上调和下调.
丰富分析表明,差异表达的circRNAs的点参与了BPH反应途径.
一个概念模型被提出,其中circRNAs通过父母基因和竞争的内源RNA (ceRNA) 网络参与大米防御.

结论:

米circRNAs在植物对抗BPH的防御调节网络中发挥着重要作用.
这些发现提供了对米-BPH相互作用背后的分子机制的洞察力,以及对抗性育种的潜在目标.

关键词:

这是一种IR56米.尼拉帕尔瓦塔 (尼拉帕尔瓦塔) 装饰品循环RNAs (循环RNAs) 是一种循环RNA.米-BPH相互作用

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