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双向比较揭示了Salmonella enterica和植物之间的相互作用中的功能模式

Min Han^1,2, Azhar A Zarkani¹, Yongming Duan¹

¹Julius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11/12, 38104 Braunschweig, Germany.

Plants (Basel, Switzerland)

|February 10, 2024

概括

沙门氏菌 (Salmonella enterica) 持续存在于像阿拉比多普西斯,生菜和西红等植物上. 病原体和植物都在这种相互作用期间动态调整它们的基因表达和新陈代谢.

科学领域:

微生物学微生物学
植物科学植物科学
食品安全食品安全

背景情况:

植物可以携带人类病原体沙门氏菌.
之前关于沙门氏菌与植物相互作用的研究使用了各种不同的实验条件,阻碍了直接比较.
了解这些相互作用对于食品安全和农业实践至关重要.

研究的目的:

根据统一的实验方法,研究沙门氏菌虫与植物的相互作用.
为了比较不同沙门氏菌血清体 (Typhimurium,Senftenberg) 的持久性和植物反应,在阿拉比多普西斯,生菜和西红中.
分析沙门氏菌和宿主植物在相互作用期间的转录基因反应.

主要方法:

用沙门氏菌 (Salmonella enterica) 菌类Typhimurium和Senftenberg接种阿拉比多普西斯,生菜和西红.
评估沙门氏菌的持久性和植物症状的发展.
对宿主植物 (番茄,阿拉比多普西斯,生菜) 和沙门氏菌的转录组分析.
来自不同植物物种和沙门氏菌株的转录基因数据的比较分析.

主要成果:

在所有测试的植物物种中,Salmonella enterica serovar Typhimurium表现出增强的持久性.
植物的反应因物种而异,观察到与病变发生有关的基因的上调调节.
番茄在24小时内对沙门氏菌表现出动态和特定的转录基因反应.
植物和沙门氏菌都表现出适应性变化:植物调整了新陈代谢,而沙门氏菌改变了细胞生物合成和新陈代谢.

结论:

沙门氏菌与植物的相互作用涉及两种生物体的动态和特定物种反应.
沙门氏菌持续存在强调了需要改善食品安全措施的必要性.
这项研究为了解沙门氏菌与植物的共同适应提供了一个比较框架.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.沙门氏菌是一种沙门氏菌.这就是Tn-Seqq.菜菜菜菜在这里,我们可以看到茄,番茄,番茄.转录组 (transcriptome) 是一个转录组.

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