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候选减弱活牛冠状病毒疫苗的特征和尖端基因分析

Gyu-Nam Park¹, SeEun Choe¹, Sok Song¹

¹Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.

Animals : an open access journal from MDPI

|February 10, 2024

概括

一种牛冠状病毒 (BCoV) 菌株通过细胞培养通道被减弱. 由此产生的KBR-1-p120菌株在小牛中显示病原性降低,表明作为活体BCoV疫苗候选人的潜力.

科学领域:

兽医病毒学兽医病毒学
传染性疾病传染性疾病
疫苗开发疫苗开发

背景情况:

牛冠状病毒 (BCoV) 在畜牧业造成重大经济损失,主要是通过小牛腹.
该KBR-1菌株是GIIa组BCoV,从小牛腹样本中分离出来.

研究的目的:

为了减弱BCoV KBR-1菌株的潜在疫苗开发.
在新生小牛中评估减弱的BCoV KBR-1-p120菌株的致病性和安全性.

主要方法:

在HRT-18和MDBK细胞中对BCoV KBR-1菌株进行持续的亚培养,以实现衰减.
用减弱的KBR-1-p120菌株和经过较少的KBR-1-p10菌株对新生小牛进行口服接种.
评估临床症状 (腹得分),便和组织中的病毒RNA检测,以及连续通道后的致病性.

主要成果:

经过120次穿越后,KBR-1-p120菌株在尖基因中表现出13种氨基酸突变,并在MDBK细胞中显示出细胞致病作用.
接种KBR-1-p10的小牛在便和肠道中显示轻度腹和可检测的BCoVRNA.
接种KBR-1-p120的小牛在便和肠道中含有很少的病毒RNA,没有观察到腹. 随后的通道并没有恢复病原性.

结论:

KBR-1-p120菌株显著减弱,在新生小牛中缺乏致病性.
减弱的KBR-1-p120菌株具有作为活牛冠状病毒疫苗候选人的潜力.

关键词:

这是一个BCoV.在 CPE 中,我们可以使用 CPE.一只小牛犊子突变是一种突变.疫苗疫苗疫苗疫苗

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