对关键模块和枢纽基因影响胸肌主要肌肉IMP内容的探索 基于WGCNA
Xinxin He1, Jinmei Xu1, Yanan Liu1
1College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Animals : an open access journal from MDPI
|February 10, 2024
概括
研究人员确定了调节肉肌肉中素单酸盐 (IMP) 含量的关键基因,这对乌玛米风味和经济价值至关重要. 这项研究揭示了影响IMP水平的分子网络,以提高质量.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 伊诺单酸盐 (IMP) 显著影响肌肉的乌玛米味道和经济价值.
- 调节肌肉中IMP含量的分子机制尚不清楚.
- 了解IMP法规对于改善肉质量至关重要.
研究的目的:
- 确定影响广德羽毛腿胸部大肌肉IMP含量的关键调控因素.
- 阐明与IMP水平相关的分子调节网络.
主要方法:
- 来自120天大的的20个胸部主要肌肉样本的转录组测序.
- 权重基因同表达网络分析 (WGCNA) 以确定与IMP内容相关的基因模块.
- 使用STRING数据库对枢纽基因进行蛋白质-蛋白质相互作用 (PPI) 网络分析.
主要成果:
- 总共分析了16344个基因,形成了20个共同表达模块.
- "紫色模块"与IMP含量有显著的负相关性 (R = -0.51,p = 0.02).
- 在紫色模块中发现了68个枢纽基因,其中TNNI1,MYOZ2,MYL2和MYL3被突出显示为参与特定信号通路的中央调节者.
结论:
- 确定了关键的基因和通路 (依赖ATP的活动,cAMP,cGMP-PKG信号传递) 调节肌肉中的IMP含量.
- 提供了对影响IMP水平的基因表达和监管网络的宝贵见解.
- 这些发现有助于理解和潜在地提高肉的风味和质量.
相关概念视频
Canonical Wnt Signaling Pathway
8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Combinatorial Gene Control
8.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.3K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
General Transcription Factors
5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K


