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在体外成熟期间补充eupatilin 通过调节氧化应激和内质网膜应激来改善猪卵细胞发育能力

Jing Wang¹, Ying-Hua Li¹, Rong-Ping Liu¹

¹Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529000, China.

Animals : an open access journal from MDPI

|February 10, 2024

概括

欧巴林补充剂改善了猪卵细胞成熟和胚胎发育. 这种天然化合物降低了氧化和内质网膜压力,增强了体外整体发育潜力.

科学领域:

生殖生物学生殖生物学
细胞压力生物学
生物化学生物化学

背景情况:

欧巴提林是一种来自Artemisia植物的黄类化合物,具有抗亡,抗氧化和抗炎性质.
欧巴林对猪卵细胞在氧化和内分泌网膜应激下成熟的影响仍然在很大程度上未被探索.

研究的目的:

调查eupatilin对猪卵细胞成熟和随后胚胎发育的保护作用.
为了确定eupatilin的最佳度,以改善体外成熟和 partenogenetic 激活.

主要方法:

在实验室中,猪卵细胞在不同度的eupatilin中成熟.
评估卵细胞成熟,胚胎发育,反应性氧物种 (ROS) 水平,谷氨生产,细胞亡,DNA损伤和内细胞网膜 (ER) 压力.
使用SPSS软件进行统计分析.

主要成果:

0.1μM欧巴林显著增强极体挤出和胚胎细胞形成率.
欧巴提林补充剂降低了ROS水平,增加了谷氨酸的产生.
治疗减少了细胞亡,DNA双链断裂和内细胞网膜压力标志物.

结论:

在体外成熟过程中补充0.1μM的eupatilin可以优化猪卵细胞的成熟和胚胎发育.
欧巴林减轻了氧化和内网膜应激对卵细胞质量的负面影响.
这项研究强调了eupatilin作为辅助生殖技术中有益的添加剂的潜力.

关键词:

欧帕提林林是什么意思在体外成熟的成熟.卵子细胞卵子细胞氧化应激是一种氧化应激.

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