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预测性评估奥克拉托辛A对氧化应激参数的影响以及使用神经网络的酵母的发酵能力
Željko Jakopović1, Davor Valinger2, Karla Hanousek Čiča3
1Laboratory for General Microbiology and Food Microbiology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia.
Foods (Basel, Switzerland)
|February 10, 2024
概括
奥克拉托克素A (OTA) 影响葡萄酒酵母发酵和氧化应激. 虽然最初抑制了乙醇生产,但24小时后的OTA没有负面影响,并且影响了谷氨和甲水平.
科学领域:
- * 微生物学与发酵科学
- * 生物化学和氧化应激分析
- * 计算生物学和预测建模
背景情况:
- *Ochratoxin A (OTA) 是一种可以影响食品生产过程的真菌毒素.
- * 葡萄酒酵母发酵对于乙醇的生产和质量至关重要.
- *像谷氨 (GSH) 和甲 (MDA) 这样的氧化应激参数是细胞健康的关键指标.
研究的目的:
- * 调查不同度的奥克拉托辛A (OTA) 对葡萄酒酵母发酵和氧化应激的影响.
- * 利用人工神经网络 (ANN) 来预测OTA对酵母发酵和氧化应激的影响.
- * 分析OTA对葡萄糖利用和葡萄酒酵母乙醇生产的影响.
主要方法:
- * 葡萄酵母对不同度的OTA进行实验暴露.
- * 测量乙醇生产,谷氨 (GSH) 和麦隆迪化 (MDA) 度.
- *人工神经网络 (ANN) 的应用用于数据分析和预测.
主要成果:
- *OTA (4μg mL-1) 最初在12小时后抑制了乙醇生产,但在24小时后没有显示任何负面影响.
- *低OTA度会增加谷氨 (GSH) 水平,而高度会影响马隆迪甲 (MDA) 水平.
- *ANN模型显示了量化实验数据和预测未经测试的OTA度的结果的潜力.
结论:
- *OTA对葡萄酒酵母乙醇生产具有复杂的,时间依赖的影响.
- *OTA以度依赖的方式影响关键的氧化应激生物标志物 (GSH和MDA).
- *人工神经网络是预测真菌毒素对酵母发酵和应激反应的影响的宝贵工具.
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