人体体内癌症相关变化的蛋白质组分析
Victor Paromov1, Vladimir N Uversky2, Ayorinde Cooley3
1Meharry Proteomics Core, RCMI Research Capacity Core, School of Medicine, Meharry Medical College, Nashville, TN 37208, USA.
International journal of molecular sciences
|February 10, 2024
概括
内在失序蛋白 (IDP) 在细胞调节和癌症中至关重要. 这项研究揭示了人类乳腺癌"展开"的显著变化,突出了IDPs.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 生物化学 生化学
- 癌症生物学 癌症生物学
背景情况:
- 许多蛋白质缺乏稳定的3D结构,作为内在无序蛋白质 (IDPs) 或包含内在无序蛋白质区域 (IDPRs) 起作用.
- IDPs/IDPRs对于分子识别和信号传导至关重要,构成了被称为"unfoldome"的人类蛋白质组的重要组成部分.
- 了解展开在乳腺癌等疾病中的作用对于识别新生物标志物和治疗点至关重要.
研究的目的:
- 为了研究人类乳腺癌的发展,通过对差异表达的IDPs进行分析.
- 探索国内流离失所者与乳腺癌中关键基因/通路之间的关系.
- 描述癌细胞中差异表达的内皮细胞的性质和疾病相关性.
主要方法:
- 利用自下而上的蛋白质组学和多维蛋白质识别技术 (MudPIT) 来分析蛋白质表达.
- 在正常 (MCF-10A) 和乳腺癌 (BT-549) 细胞系之间比较蛋白质概况.
- 对已识别的境内流离失所者进行了in silico表征和基因本体学 (GO) 注释.
主要成果:
- 在正常和癌症蛋白质组中确定了2271个蛋白质组.
- 在乳腺癌细胞中发现148种内在失序蛋白 (IDP),表达显著差异.
- 标注了140名境内流离失所者,其中65%与疾病有关,20%与癌症有关. 在不同表达的国内流离失所人口中确认了无序的地区.
结论:
- 人类乳腺癌展开组表现出高度的互动性和受损蛋白质的普遍性.
- 不同表达的内部流离失所者与各种疾病,特别是癌症有显著的关联.
- 这些发现强调了内脏癌症在乳腺癌发病过程中的重要性,并表明了它们作为诊断或治疗点的潜力.
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