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黄色种子颜色基因的识别使用大量分离RNA测序在白树的L

Yang Wang¹, Hong Lu¹, Xiang Liu¹

¹State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, China.

International journal of molecular sciences

|February 10, 2024

概括

研究人员确定了一个关键基因BjuA09PAL2,它负责子 (Brassica juncea) 的黄色种子颜色. 这一发现有助于通过菜种子的黄色种子育种来提高石油产量和质量.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业科学农业科学

背景情况:

橄油 (Brassica napus L.) 的黄色种子颜色对于提高油的产量和质量是理想的.
在B. napus中没有自然存在的黄色种子基因型,因此需要对子 (Brassica juncea L.) 等相关物种进行研究.
在B. juncea中黄色种子颜色的分子基础在很大程度上仍然没有被描述.

研究的目的:

为了确定控制黄色种子颜色的候选基因在Brassica juncea.
了解B. juncea.黄色种子特征背后的分子机制.
为增强黄种子菜种子育种提供基础.

主要方法:

大量分离RNA测序 (BSR-Seq) 在BC9种群中进行,该种群来自黄色和棕色末种子之间的交叉.
使用已识别的单核酸多态 (SNP) 和INDEL进行了定量特征位置 (QTL) 分析.
蛋白质对蛋白质网络分析被用来识别在黄类生物合成途径中的枢纽基因.

主要成果:

鉴定了511个差异表达基因 (DEG),其中许多参与了烯和黄生物合成.
在染色体A09.09上发现了两个显著的QTLs (qSCA09-3和qSCA09-7).
编码氨酸酶的BjuA09PAL2被确定为最可能的黄色种子颜色候选基因,SNP在黄色和棕色种子B. juncea基因组之间一致.

结论:

BjuA09PAL2是一种新型候选基因,与Brassica juncea的黄色种子颜色有关.
这一发现对标记辅助选择和黄种子特征在菜种子育种中的遗传改进具有重大意义.
了解种子颜色的遗传基础可以加速改良油作物的发展.

关键词:

B. 叶树 (Juncea) 是一种植物.这就是BSR-Seqq.DEGs 是一个DEG.在 QTL 时段,你会得到 QTL.在SNP中,SNP是SNP.黄色种子的种子是黄色的

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