DNA甲基化和染色质可访问性影响 常见鱼 (Cyprinus carpio) 中亚基因组表达主导地位
Shuang-Ting Yu1,2, Ran Zhao1, Xiao-Qing Sun1
1Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs and Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China.
International journal of molecular sciences
|February 10, 2024
概括
表观遗传修饰,特别是DNA甲基化和染色质可访问性,影响普通鱼的基因表达. 与A亚基因组相比,B亚基因组显示出更高的染色质可访问性,导致基因表达的增加.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 水产养殖是水产养殖的一种方式.
背景情况:
- 基因甲基化和染色质可访问性是基因表达的关键调节者.
- 它们在allotetraploids亚基因组表达优势中的作用尚不清楚.
研究的目的:
- 为了研究表观遗传修饰在亚基因组表达主导地位的功能在allotetraploid常见鱼.
- 分析转录组,DNA甲基化和肝脏和肌肉组织中的染色质可访问性.
主要方法:
- 对转录基因数据的全面分析.
- 评估促进体和基因体区域的DNA甲基化模式.
- 使用ATAC-seq或类似方法进行染色体可访问性分析.
主要成果:
- 观察到A和B亚基因组之间的DNA甲基化水平有显著差异,但与偏差表达没有相关.
- B亚基因组显示出更大的染色质可访问性和更高的开放染色质区域的频率.
- 具有增强色素可访问性的基因,特别是在B亚基因组中,表达水平明显更高.
结论:
- 染色质可访问性,而不是DNA甲基化,似乎是普通鱼亚基因组表达主导的主要驱动因素.
- 这项研究提供了关于全四鱼基因表达的表观遗传调节的见解.
相关概念视频
01:46Epigenetic Regulation
31.1K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.1K
02:30Genomic Imprinting and Inheritance
34.4K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
34.4K
02:32Position-effect Variegation
6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
02:32Histone Modification
13.3K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.3K
02:35Chromatin Position Affects Gene Expression
23.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.3K
03:17Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K