后生物酸盐减轻了急性乙醇暴露引起的肠粘膜干扰
Mohamed Tausif Siddiqui1,2, Yingchun Han2, David Shapiro2
1Department of Gastroenterology, Hepatology and Nutrition, Digestive Disease Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
International journal of molecular sciences
|February 10, 2024
概括
通过tributyrin输送的后生物丁酸盐保护了肠道屏障的完整性,并在急性乙醇暴露期间减少了炎症. 这突显了丁酸盐在控制酒精诱导的肠道损伤和失能症方面的潜力.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 急性乙醇暴露可以破坏肠道屏障的完整性并促进炎症.
- 通常由抗生素或乙醇诱导的肠道功能障碍症会加剧这些影响.
- 后生物制剂,如丁酸盐,显示在减轻酒精诱导的肠道损伤的潜力.
研究的目的:
- 调查丁酸盐在急性乙醇暴露期间对肠道细菌,上皮质完整性和微血管内皮细胞激活的保护作用.
- 评估酸盐前药物支柱氨酸对小鼠和人类肠道微血管内皮细胞 (HIMECs) 的肠道健康的影响.
- 评估tributyrin在调节肠道失调和乙醇挑战的背景下调节免疫反应中的作用.
主要方法:
- 挑战小鼠以乙醇,有或没有补充tributyrin,和一些接受了克林达米辛预处理.
- 分析了肠道屏障的完整性,血内毒素水平,状细菌和膜自身树突细胞.
- 初级人类肠道微血管内皮细胞 (HIMECs) 暴露在乙醇和脂多糖化物 (LPS) 中,有或没有甲酸盐.
主要成果:
- 在乙醇和抗生素暴露期间,丁酸盐补充剂保留了产生酸盐的微生物.
- 丁氨酸减轻了乙醇诱导的阴上皮质屏障破坏和降低了血内毒素.
- 酸丁酸和酸丁酸减弱了内皮激活 (MAdCAM-1表达) 并促进了耐受性树突细胞反应.
结论:
- Tributyrin 补充剂可以保护小肠表皮和微血管屏障免受乙醇诱导的损伤.
- 丁酸盐调节微血管内皮激活和树突细胞功能,为与酒精有关的肠道问题提供治疗策略.
- 这些发现表明,丁酸盐在治疗肠道失调症和相关炎症状况方面具有潜力.
更多相关视频
05:12Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder
Published on: June 23, 2023
906
08:37Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer
Published on: November 15, 2024
325
相关概念视频
02:12Solvating Effects
7.8K
An understanding of the solvating effect helps rationalize the relation between solvation and acidity of the compound. In addition, this also explains the relative stability of conjugate bases for compounds with different pKa values. This lesson details, in-depth, the principle of solvating effects. The strength of an acid and the stability of its corresponding conjugate base are determined using pKa values. This observed relationship is a consequence of solvation, which is the interaction...
7.8K
01:25Microbes in Beverage Production
298
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
298
01:22Probiotics
296
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
296
01:18Functions of the Gut Microbiota
233
The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
233
01:22Gut-Brain Axis
222
The gut–brain axis is a bidirectional communication system that connects the gastrointestinal tract and the brain. This interaction is mediated through multiple pathways, including the vagus nerve, hormonal signals, immune responses, and chemical messengers produced by gut microbes.Microbial Contributions to Brain FunctionGut microbiota contributes significantly to brain function by producing neuroactive compounds. These include neuroactive compounds that influence neurotransmitters such...
222