低密度中性粒细胞有助于2型糖尿病患者的亚临床炎症
Benjamin L Dumont1,2, Paul-Eduard Neagoe1, Elcha Charles1,2
1Research Center, Montreal Heart Institute, Montreal, QC H1T 1C8, Canada.
International journal of molecular sciences
|February 10, 2024
概括
2型糖尿病患者的低密度中性粒细胞 (LDNs) 和中性粒细胞外细胞陷 (NETs) 增加,导致炎症. 这些发现突出了LDN和NET作为糖尿病管理中的潜在生物标志物和治疗目标.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 病理生理学 病理生理学
背景情况:
- 2型糖尿病 (T2D) 与慢性低度炎症有关.
- 中性粒细胞,特别是低密度中性粒细胞 (LDNs),在炎症过程中发挥作用.
- 中性细胞外细胞陷 (NETs) 涉及到各种病理.
研究的目的:
- 调查T2D患者中高密度中性粒细胞 (HDNs) 和LDNs的数量和功能.
- 评估在T2D中循环的NETs生物标志物和促炎性细胞因子水平.
- 评估来自T2D患者的LDN的NET形成能力和粘附性质.
主要方法:
- 光激活细胞分类 (FACS) 和流细胞测量用于中性粒细胞子集量化.
- 用于测量循环炎症标记物 (IL-6,IL-8,CRP) 和NETs生物标记物 (H3Cit,MPO,MPO-DNA) 的ELISA.
- 对焦显微镜用于NET形成量化和光学显微镜用于中性粒细胞粘附度评估.
主要成果:
- 与健康志愿者 (HVs) 相比,T2D患者的LDN数量显著增加 (160%).
- 在T2D患者中观察到高水平的NETs生物标志物 (H3Cit,MPO,MPO-DNA) 和促炎细胞因子 (IL-6,IL-8,CRP).
- 从T2D患者中分离出来的LDN显示了增加的NET产量和对人类细胞外基质 (hECM) 的增强粘附.
结论:
- 患有T2D的患者在循环中的LDN和NET相关生物标志物的水平上升.
- 来自T2D患者的LDN表现出增强的抗炎活动,包括增加NET形成和粘附.
- 这些发现表明LDN和NET是T2D炎症的关键因素,并代表潜在的治疗点.
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