不同组织和不同类型的骨肌肉老化期间MAPKs活动的异步模式
Nechama Gilad1,2, Manju Payini Mohanam2,3, Ilona Darlyuk-Saadon2
1Department of Biological Chemistry, The Institute of Life Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
International journal of molecular sciences
|February 10, 2024
概括
这项研究表明,像p38α,JNK和ERK这样的线粒激活蛋白激酶 (MAPK) 不会在所有组织和肌肉类型中随着衰老而变化. 衰老的衰老 衰老的衰老
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 衰老研究研究 衰老研究
- 生理学 生理学 生理学
背景情况:
- 包括p38α,JNK和ERK在内的基激活蛋白激酶 (MAPK) 参与细胞衰老过程.
- 以前的研究表明p38α促进骨肌肉衰老,但其在不同肌肉类型和其他器官中的作用仍然不清楚.
- 在衰老过程中MAPK活性的特定模式没有得到充分的记录.
研究的目的:
- 研究p38α,JNK和ERK在老化期间在各种小鼠组织和骨肌肉中的活动模式.
- 为了确定MAPK活动是否在不同的生物环境中随着年龄变化而变化均.
- 探索MAPK活动,细胞外矩阵 (ECM) 组件和四头肌肌中的衰老标志物之间的关系.
主要方法:
- 来自不同年龄 (1至24个月) 的小鼠的多个器官和特定的骨肌肉 (独身肌,前肌,四头肌,腹半肌,EDL) 中的化 (活性) p38α,Erk1/2和JNK水平的量化.
- 从老化小鼠的四头肌的RNA测序 (RNA-seq) 分析.
- 对细胞循环抑制剂和衰老相关蛋白质mRNA表达的分析.
主要成果:
- 在大多数研究的组织和骨肌肉中,MAPK活性在衰老期间通常保持稳定.
- 在年轻组织中,p38α活性往往更高,除了四头骨和肺部,老年小鼠 (≥13个月) 的活性增加.
- 陈旧的四头骨和肺部显示JNK和ERK活性水平升高,以及四头骨中ECM和ERK信号通路基因的下调. 已知的衰老标志物没有升高.
结论:
- 衰老期间的MAPK激活模式是特定于组织和肌肉类型的.
- 衰老过程本身表现出显著的组织和细胞特异性特征,而不是普遍的分子特征.
- 这些发现挑战了特定MAPKs在衰老中的普遍作用,并突出了与年龄相关的分子变化的复杂性.
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