在Cydia pomonella (Lepidoptera: Tortricidae) 中的三种一般气味结合蛋白2基因的分子和功能表征
Yanan Zhou1,2, Cong Huang2,3, Guanjun Fu1,2
1College of Plant Health & Medicine, Qingdao Agricultural University, Qingdao 266109, China.
International journal of molecular sciences
|February 10, 2024
概括
在鱼 (Cydia pomonella) 中,三种通用气味结合蛋白2 (GOBP2) 基因表现出不同的功能. CpomGOBP2a和CpomGOBP2b与特定化合物结合,而CpomGOBP2c没有结合,表明嗅觉检测的功能差异.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 一般的气味结合蛋白 (GOBPs) 对于检测昆虫中的植物挥发物和激素至关重要.
- codling moth (Cydia pomonella) 的基因组含有重复的GOBP2基因,表明功能专业化.
研究的目的:
- 为了研究三种Cydia pomonella GOBP2基因 (CpomGOBP2a,CpomGOBP2b,CpomGOBP2c) 的功能分歧.
- 为了确定GOBP2蛋白质的联结特异性.
主要方法:
- 定量实时PCR (qRT-PCR) 用于基因表达分析.
- 重组GOBP2蛋白的蛋白质表达和净化.
- 光竞争性结合试验测试以测试与宿主植物挥发性物质和鱼的结合亲和力.
主要成果:
- CpomGOBP2a和CpomGOBP2b在天线中表达高,而CpomGOBP2c在翅膀中表达高.
- CpomGOBP2a强烈结合于乙乙烯酸乙,梨,鱼和日拉尼拉.
- CpomGOBP2b特别与梨结合,而CpomGOBP2c并没有与任何测试化合物结合.
结论:
- 这三种Cydia pomonella GOBP2基因在带结合中表现出功能分歧.
- 这些发现提供了对嗅觉基因功能的演变和潜在的害虫控制策略的见解.
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