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基于光的蛋白质稳定性监测-一篇评论

Negin Gooran1, Kari Kopra1

  • 1Department of Chemistry, University of Turku, Henrikinkatu 2, 20500 Turku, Finland.

International journal of molecular sciences
|February 10, 2024
PubMed
概括

本综述探讨了研究蛋白质稳定性的无标签方法,重点关注基于光的技术,如热转移试验 (TSA),并将其与异热化学变质 (ICD) 进行比较. 这些方法对于了解蛋白质折叠和功能至关重要.

关键词:
吉布斯的自由能量是自由的.蛋白质 试探器塞浦路斯SYPRO的色是色的差分扫描计 (DSF) 是一种不同的扫描计.外部染料是一种外在的染料.光是一种光.内在的光是一种内在的光.异热化学去化 (ICD) 是一种方法.化的温度 (Tm)蛋白质的稳定性 蛋白质的稳定性热转移试验 (TSA) 是一种热转移试验.尿素 urea 的使用方法

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