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前列腺素F2α 影响时钟基因表达和小鼠行为周期

Yuya Tsurudome¹, Yuya Yoshida², Kengo Hamamura²

¹Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

International journal of molecular sciences

|February 10, 2024

概括

缺乏前列腺素F受体 (Ptgfr) 的小鼠表现出改变的生物节奏和时钟基因表达. 这表明PGF2α和Ptgfr向药物可能会影响昼夜周期.

科学领域:

时间生物学时间生物学
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

前列腺素及其受体影响基因表达.
前者对前列腺素F受体 (Ptgfr) 在生物节律中的作用是未知的.

研究的目的:

研究Ptgfr与昼夜节律之间的关系.
确定Ptgfr缺乏对时钟基因表达的影响.

主要方法:

在恒定的黑暗条件下测量Ptgfr-knockout (KO) 和野生类型小鼠的运动运动活动周期.
在上核和肝脏中分析时钟基因表达 (Cry1,Cry2,Per2).
在体外实验中使用PTGFR激动剂和Per2::luciferase记者测定.

主要成果:

在恒定的黑暗条件下,Ptgfr-KO小鼠表现出更长的运动运动周期.
在Ptgfr-KO小鼠的上体核中观察到减少的加密染色体1 (Cry1) 表达.
在Ptgfr-KO小鼠的肝脏中发现了Cry1,Cry2和Period2 (Per2) mRNA水平的显著变化.
血前列腺素F2α (PGF2α) 水平在野生型小鼠中表现出昼夜节律.
以时间依赖的方式调节P2::luc活动的PTGFR激动剂添加.

结论:

前列腺素F受体 (Ptgfr) 参与调节生物节律.
血PGF2α的循环节律可能会驱动时钟基因表达.
针对PGF2α和Ptgfr的药物是生物钟的潜在调节剂.

关键词:

昼夜时钟是生物周期的时间表.运动机动活动运动机动活动鼠标行为鼠标行为的前列腺素F2αα.上的核是一个上的核.

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