YTHDF1在乳腺癌预后,免疫力和ceRNA网络中的监管参与
Wenting Luo1,2, Youjia Zhou1, Jiayang Wang3
1Department of Pathophysiology, Medical College, Nanchang University, 461 Bayi Road, Nanchang 330006, China.
International journal of molecular sciences
|February 10, 2024
概括
YTHDF1蛋白对乳腺癌的进展和免疫微环境至关重要. 它的调控网络,包括ceRNA,为乳腺癌免疫治疗提供了潜在的治疗点和生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 甲基氨酸RNA结合蛋白1 (YTHDF1) 是一种m6A读者,与乳腺癌的发展及其免疫微环境有关.
- 竞争的内源RNA (ceRNA) 网络因其在癌症调节中的作用和瘤基因治疗中的潜力而受到关注.
研究的目的:
- 研究YTHDF1表达,乳腺癌临床病理学和患者存活率之间的关系.
- 探索YTHDF1在瘤突变负担,微卫星不稳定性和瘤微环境中的作用.
- 阐明乳腺癌中YTHDF1和ceRNA网络之间的相互作用.
主要方法:
- 对TCGA和PrognoScan数据集的分析,以将YTHDF1表达与生存率和临床病理学相关联.
- 在乳腺癌组织中对YTHDF1表达的西方斑点验证.
- 探索YTHDF1与瘤突变负担,微卫星不稳定性和免疫微环境组件的功能关联.
- 构建和分析涉及YTHDF1和miR-378g.g.的新型ceRNA网络
主要成果:
- YTHDF1在乳腺癌组织中显著表达.
- YTHDF1的表达与乳腺癌的进展相关,并可能影响瘤的免疫微环境.
- 确定了三个新的ceRNA网络 (DR4-AS1-miR-378g-YTHDF1,HCG9-miR-378g-YTHDF1, taurine上调调节的1-miR-378g-YTHDF1).这些网络中,有三种新型的ceRNA网络,分别为DR4-AS1-miR-378g-YTHDF1和HCG9-miR-378g-YTHDF1.
- 发现miR-378g可以抑制YTHDF1的表达,miR-378g/YTHDF1轴影响MDA-MB-231细胞增殖.
结论:
- YTHDF1是乳腺癌中关键的m6A调节蛋白,在免疫微环境中起着重要作用.
- YTHDF1可以作为预后生物标志物和乳腺癌免疫治疗的潜在治疗标.
- 已确定的ceRNA网络,特别是涉及miR-378g和YTHDF1,突出了影响乳腺癌细胞生长的新型调节轴.
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