小鱼体特异性RNA12 通过调节膀癌中的细胞外基质信号来促进癌症发生
Qinchen Lu1,2,3, Jiandong Wang1,2,3, Yuting Tao2
1Department of Urology, Guangxi Medical University Cancer Hospital, Nanning 530021, China.
Cancers
|February 10, 2024
概括
小鱼体特异性RNA 12 (SCARNA12) 在膀癌 (BLCA) 中被上调,促进瘤生长和转移. 针对SCARNA12及其与H2AFZ的相互作用,可能为BLCA提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 小卡哈尔体特异性RNAs (scaRNAs) 正在成为生理和病理过程中的关键参与者.
- 包括SCARNA12在内的scaRNAs在癌症发展中的特定作用在很大程度上仍未被探索.
- 了解scaRNA功能,可以了解新的癌症机制.
研究的目的:
- 研究SCARNA12在膀癌 (BLCA) 中的作用和机制.
- 探索SCARNA12作为BLCA中的潜在生物标志物和治疗点.
- 阐明SCARNA12在BLCA进展中影响的分子通路.
主要方法:
- 对SCARNA12表达和BLCA临床意义的TCGA,GEO和GTEx数据集的分析.
- 在BLCA细胞系和组织中使用qPCR和in situ杂交验证SCARNA12表达.
- 通过RNA-seq,CyTOF,CRISPR/Cas9介导的淘汰,体外/体内测试和ChIRP实验来评估SCARNA12的功能和机制.
主要成果:
- 在BLCA组织和细胞系中,SCARNA12表达显著上调.
- 斯卡尔纳12 Knockdown 抑制BLCA细胞的增殖,迁移,入侵和瘤生长,同时诱导细胞亡和细胞循环停止.
- SCARNA12与H2AFZ相互作用,调节转录并影响细胞外基质 (ECM) 信号通路.
结论:
- 通过ECM信号调制,SCARNA12在BLCA进展中起着显著的瘤作用.
- 斯卡纳12-H2AFZ相互作用对BLCA致癌和进展至关重要.
- SCARNA12具有作为BLCA的诊断生物标志物和治疗点的潜力.
更多相关视频
11:02Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
21.3K
12:14CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
Published on: June 2, 2022
2.6K
相关概念视频
02:38The Ras Gene
6.2K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
6.2K
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:22Microtubules in Signaling
1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
01:24Small GTPases - Ras and Rho
4.0K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
4.0K
01:32RNA Splicing
56.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.4K