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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 3, 2025

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
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实体细胞成像采用单一拍摄的通用通路宽视场反射数字全息显微镜.

Manoj Kumar1,2, Takashi Murata3, Osamu Matoba1,2

  • 1Department of Systems Science, Graduate School of System Informatics, Kobe University, Rokkodai 1-1, Nada, Kobe 657-8501, Japan.

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|February 10, 2024
PubMed
概括

一个新的数字全息显微镜 (DHM) 系统提供了细胞和表面的广场,单次拍摄,无标签成像. 这种具有成本效益,对振动不敏感的技术增强了生物和微表面调查.

关键词:
数字全息显微镜的数字全息显微镜.活细胞成像 活细胞成像定量阶段成像成像技术

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Last Updated: Jul 3, 2025

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科学领域:

  • 光学和光子学 在光学和光子学.
  • 生物医学成像技术 生物医学成像技术
  • 显微镜的使用方法

背景情况:

  • 使用数字全息显微镜 (DHM) 的定量相成像 (QPI) 是一种重要的无标签技术.
  • 在分析未染色的活细胞和反射表面方面,DHM非常出色.

研究的目的:

  • 引入一种全新的广视场,单射,通道离轴反射DHM系统.
  • 证明其对生物和微表面样本定量相位成像的能力.

主要方法:

  • 开发了一个紧的,稳定的,和成本效益的共同路径离轴反射DHM配置.
  • 利用一次性采集来实现高效的相位成像.
  • 用美国空军的分辨率测试目标和活植物细胞测试了系统.

主要成果:

  • 实现了反射和透明样本的高质量定量相位成像.
  • 由于其通用路径设计,证明了系统对外部振动的强度.
  • 验证了该系统适用于生物标本和微表面计量学的适用性.

结论:

  • 拟议的DHM配置为QPI提供了一种多功能,稳定和具有成本效益的解决方案.
  • 它为无标签的生物细胞研究和微型表面的精确光学计量提供了显著的优势.