基于保存冷时间和温度的肠道组织机械性质的差异
Gregory S Hirst1, Sunandita Sarker2, Benjamin S Terry1
1Brigham Young University, United States.
Journal of the mechanical behavior of biomedical materials
|February 10, 2024
概括
结猪小肠组织会增加其硬度,无论温度或持续时间如何. 与新鲜组织相比,冷的组织表现出更高的应力和应变能量,这可能是由于影响组织结构的冰形成.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 机械工程 机械工程
背景情况:
- 了解冷保存对生物组织的影响对于组织银行和再生医学至关重要.
- 组织的机械性能可能会因储存条件而显著改变,从而影响其适用于各种应用的适用性.
- 以前的研究已经探讨了冷保存效应,但在各种冷条件下对猪肠道组织的具体数据是有限的.
研究的目的:
- 研究不同冷时间和温度对猪小肠组织机械性能的影响.
- 在冷保存后量化压力和应变能量的变化.
- 为了确定特定的冷参数 (温度和持续时间) 是否对组织力学产生不同的影响.
主要方法:
- 猪小肠组织样本被分为五组:新鲜 (对照组),在28.9°C冷7天,在62.2°C冷7天,在28.9°C冷30天,在62.2°C冷30天.
- 组织样本被储存在PBS中,解,并接受双轴机械测试以测量应力和张力能量.
- 第一个0.2张的Piola-Kirchhoff应力是使用厚度和力数据计算的.
主要成果:
- 与新鲜组织相比,所有冷组织群体的压力和应变能量都呈现出统计学上显著的增加.
- 不同的结温度 (-28.9°C与-62.2°C) 或持续时间 (7天与30天) 之间没有观察到机械性质的统计学上显著差异.
- 平均周围应力值在22.3kPa (新鲜) 到46.5kPa (在-62.2°C冷7天) 之间.
结论:
- 冷保存,无论测试的温度和持续时间,都会增加猪小肠组织的刚性.
- 观察到的硬度增加可能归因于冰晶的形成,导致离子度的改变和糖氨基甘氨酸与原纤维的相互作用.
- 这些发现对肠道组织在研究和临床应用中的保存和使用有影响.
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