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亚MIC的万科米辛通过降低蛋白质糖化的调节来增强万科米辛中间体黄金葡萄球菌的抗生素耐受性

Yi Yang¹, Li Tan¹, Siyuan He²

¹Department of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering Under the Educational Committee in Chongqing, Army Medical University (Third Military Medical University), Chongqing 400038, China.

Microbiological research

|February 10, 2024

概括

暂时暴露于万科米会提高黄金葡萄球菌 (Staphylococcus aureus) 的细菌耐受性. 蛋白质化 (Ksucc) 修饰,特别是在MurA中,是一种新的机制,可以调节这种万科米辛耐受性.

科学领域:

微生物学微生物学
分子生物学分子生物学
抗生素耐药性抗生素耐药性

背景情况:

细菌对抗生素的耐受性,是耐药性的前体,是由暂时的药物暴露驱动的.
了解耐受性机制对于打击抗生素耐药性至关重要.

研究的目的:

为了研究在米素中间体黄金葡萄球菌 (VISA) 菌株中米素耐受性背后的分子机制.
探索蛋白质化 (Ksucc) 在调节万科米辛耐受性的作用.

主要方法:

培养VISA菌株,其菌素的抑制度低于最小值 (MIC).
西方斑点分析以评估蛋白质糖化水平.
基因操纵,包括基因删除 (SacobB) 和位点定向突变发生 (MurA).

主要成果:

暴露于万科米辛导致VISA菌株Ksucc水平降低.
删除SacobB影响了Ksucc的修饰,万科胺素耐受性和细胞壁合成.
在MurA中发生的突变,Ksucc修饰的向蛋白,降低了万科米辛最小抑制度 (MICs),其中一个突变变得对万科米辛敏感.

结论:

蛋白质Ksucc修饰是一种新型的调节机制,影响黄金葡萄球菌 (Staphylococcus aureus) 的万科米辛耐受性.
暴露于万科米辛会通过调节蛋白Ksucc水平影响细菌耐受性.
准Ksucc修饰途径可能为针对万科米辛中间菌株提供新的策略.

关键词:

暴露于抗生素中的抗生素.氨酸糖化素.穆拉的意思是穆拉.这就是SaCobBB.对万科米辛的耐受性

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