从铁质湖水中丰富电活性微生物 - - 注意硫酸盐降解剂!
Astolfo Valero1, Daniel A Petrash2, Anne Kuchenbuch3
1Institute of Soil Biology and Biogeochemistry, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic; Department of Ecosystem Biology, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
Bioelectrochemistry (Amsterdam, Netherlands)
|February 10, 2024
概括
从无氧,富含金属的水中培养电活性微生物是具有挑战性的. 抑制硫酸盐降解剂对于防止阳极被动化和使电气缩成为可能至关重要.
科学领域:
- 环境微生物学环境微生物学
- 电化学 电化学 电化学
- 生物地质化学生物地质化学
背景情况:
- 电活性微生物 (EAM) 在氧化还原界面驱动矿物质转化.
- 从高氧和金属梯度的环境中系统地种植EAM是很困难的.
- 来自采矿后湖泊的无毒,铁质水是EAM的独特来源.
研究的目的:
- 为了从无氧的,富含金属的开采后湖水中丰富电活性微生物.
- 调查影响电致性和微生物群落组成的因素.
- 识别挑战和策略来培养EAM从具有挑战性的环境.
主要方法:
- 浮游生物细胞从无氧铁质水中进行阳极丰富.
- 多样化的基质组成和酵母提取物添加.
- 二次丰富策略. 二次丰富策略.
- 扫描电子显微镜和能量分散式X射线光谱学.
主要成果:
- 观察到微弱的电致性 (∼5μA cm-2),取决于浮游生物细胞和形式.
- 酵母提取物减少了滞后阶段,但并没有降低电流密度.
- 丰富的社区包括硫酸盐和酸盐减少细菌,以及后来减少铁和发酵细菌.
- 在阳极上形成的富含硫和铁的聚合物阻碍了当前的生产.
结论:
- 无毒的,地质上富含金属的水可以用于弱电气缩.
- 抑制硫酸盐降解剂对于防止通过生物矿物化的阳极被动化至关重要.
- 为了成功种植EAM,必须对微生物联盟进行仔细的控制.
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