一种PilZ域蛋白与转录调节器Hink相互作用,以调节Pseudomonas aeruginosa中的VI型分泌系统
Tianfang Cheng1, Qing Wei Cheang2, Linghui Xu1
1Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.
The Journal of biological chemistry
|February 10, 2024
概括
伪omonas空气菌 伪omonas空气菌
科学领域:
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
- 微生物病原体的产生
背景情况:
- 六型分泌系统 (T6SS) 对细菌竞争和毒性至关重要.
- 通过定量检测和c-di-GMP来调节Pseudomonas aeruginosa中的T6SS是复杂的,并未完全理解.
研究的目的:
- 阐明了c-di-GMP结合蛋白PA0012调节P. aeruginosa.中T6SS基因表达的分子机制.
- 确定和描述PA0012参与T6SS监管的交互伙伴.
主要方法:
- 酵母两杂交查以确定蛋白质相互作用体.
- 同免疫沉测试以确认蛋白质与蛋白质相互作用.
- 电泳运动转移试验 (EMSA) 用于研究DNA-蛋白质结合.
- 细菌表型的分析,包括蜂群的运动性,皮约素的生产,和T6SS介导的杀死.
主要成果:
- PA0012 (更名为TssZ) 与转录调节器 HinK.直接相互作用.
- TssZ-HinK相互作用的反向调节是由c-di-GMP水平,不同于典型的PilZ域蛋白相互作用.
- TssZ 抑制了 HinK 与 pqsR 促进剂的结合,影响了定数感应.
- TssZ和HINK以PqsR依赖的方式调节P. aeruginosa毒性因子和T6SS活动.
结论:
- 通过与HINK相互作用,TssZ作为T6SS基因表达的新型抑制剂.
- 这项研究揭示了P. aeruginosa中T6SS的新调节途径,涉及TssZ,HINK和c-di-GMP.
- 这些发现提供了关于第二信使,转录调节器和细菌病原体中的分泌系统之间的复杂相互作用的见解.
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