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用热激活水中活性的诺罗病毒和MS2菌体

Marlee Shaffer¹, Kimberly Huynh², Verónica Costantini²

¹Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, IN 46556, United States.

Journal of applied microbiology

|February 10, 2024

概括

MS2菌体是人类诺罗病毒热失活的保守代用物,可能会高估感染风险. 通过PCR测量病毒RNA并不反映热处理后可行的诺罗病毒持久性.

科学领域:

食品安全食品安全
病毒学病毒学
微生物学微生物学

背景情况:

人类诺罗病毒是导致食物传播疾病的重要原因之一.
MS2菌通常被用作替代体来研究诺罗病毒失活.
对病毒失活的准确评估对公共卫生至关重要.

研究的目的:

为了比较可行的人类诺罗病毒和MS2菌的热失活动力学.
为了评估热不活化过程中病毒RNA信号的衰变.
为了确定MS2菌体的适用性,作为热无活化研究的诺罗病毒代用物.

主要方法:

人类肠道体被用于培养和分析可行的人类诺罗病毒.
使用斑块分析培养MS2菌体.
在50°C,60°C和70°C时确定热失活动力学.
使用逆转录-PCR测量病毒RNA衰变.

主要成果:

在所有测试温度 (50°C,60°C,70°C) 中,人类诺罗病毒的衰变率明显高于MS2菌体.
诺罗病毒的衰变率随温度增加,从0.22分钟-1到1.11分钟-1.1不等.
MS2菌体衰变速率从0.0065分钟-1到0.16分钟-1.1不等.
使用PCR的任何一种病毒都没有观察到RNA标位的下降,这表明分子方法低估了无活化.

结论:

MS2菌体作为人类诺罗病毒热失活的保守代用物,可能会高估感染风险.
通过PCR测量病毒RNA标位并不准确地反映热失活后可活性的人类诺罗病毒的持久性.
目前的分子方法可能低估了热处理对诺罗病毒消毒的有效性.

关键词:

热不活化热不活化热不活化人类诺罗病毒人类诺罗病毒持续性持久性替代子女可以成为替代子女.公司的可行性,可行性.

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