稳定的内子的营养依赖调节调节了生殖线线线粒体质量控制
Annabel Qi En Ng1, Seow Neng Chan1, Jun Wei Pek2,3
1Temasek Life Sciences Laboratory, 1 Research Link National University of Singapore, Singapore, 117604, Singapore.
Nature communications
|February 10, 2024
概括
禁食会导致线粒体结块,并通过增加sisR-1 RNA,阻止果的 oogenesis. 降低sisR-1水平可以恢复卵子生成并改善卵子质量,从而揭示出一种新的线粒体质量控制机制.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 线粒体是从母亲遗传的,对胚胎发育至关重要.
- 产卵生成涉及严格的生殖线线线粒体质量控制,以确保卵细胞的活力.
- 营养素的可用性对线粒体质量控制的影响仍然在很大程度上是未知的.
研究的目的:
- 为了研究营养的可用性,特别是禁食,如何影响胚胎线线粒体质量控制在 oogenesis .
- 阐明将禁食与线粒体功能障碍和 oogenesis 停止联系的分子机制.
- 探索调节这种途径以改善卵细胞质量的潜力.
主要方法:
- 使用Drosophila melanogaster作为一个模型生物.
- 研究了禁食对线粒体形态学和 oogenesis 的影响.
- 分析了基因表达的变化,重点关注DIP1-Clueless通路和sisR-1RNA.
- 使用RNA干扰 (RNAi) 来操纵sisR-1水平.
- 在干预后评估了线粒和卵细胞质量.
主要成果:
- 禁食诱导了Drosophila的线粒体凝聚和 oogenesis 停止.
- 禁食导致DIP1-Clueless通路活性降低,并增加sisR-1RNA表达.
- sisR-1 RNA 通过阻止 Porin/VDAC1 聚-ubiquitination,从而抑制p62-介导的线粒细胞衰变,从而抑制了线粒细胞衰变.
- 降低sisR-1水平或重新养恢复了线粒细胞衰变,卵细胞生成和卵细胞质量.
结论:
- 禁食通过sisR-1通路损害了生殖线线线粒体质量控制,导致 oogenesis 停止.
- 调节sisR-1水平提供了一种潜在的策略,可以在禁食条件下改善卵细胞质量.
- sisR-1通路在生殖线线线粒体质量控制中发挥着更广泛的作用,应对各种压力因素,如蛋白质缺乏,热冲击和衰老.
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