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Updated: Jul 3, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

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采集单细胞蛋白质组分析,用于量化哺乳动物细胞中多达3000种蛋白质

Yu Wang1,2,3, Zhi-Ying Guan1, Shao-Wen Shi2

  • 1Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.

Nature communications
|February 10, 2024
PubMed
概括

我们开发了一种新的单细胞蛋白质组分析 (PiSPA) 工作流程,显著增加蛋白质识别到每细胞约3000组,以获得更深入的生物学见解.

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相关概念视频

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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