相关实验视频

Updated: Jul 3, 2025

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Published on: May 3, 2024

通过NF-κB/AIM2/Caspase-1通路,GSDMD介导的烧诱导了格雷夫斯轨道病的炎症

Xiaotong Sha¹, Huijing Ye¹, Xing Wang¹

¹State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.

Experimental eye research

|February 11, 2024

概括

格雷夫斯轨道病 (GO) 患者的Gasdermin D (GSDMD) 含量升高,与疾病活性相关. 阻止GSDMD可以减少炎症,这表明它是GO的潜在治疗目标.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
眼科医生眼科眼科

背景情况:

加斯德明D (GSDMD) 执行 pyroptosis,这是一个关键的炎症过程,与自身免疫性疾病有关.
在Graves'轨道病 (GO) 中GSDMD的作用,这是一种影响眼睛的自身免疫性炎症状况,仍然未被探索.

研究的目的:

调查GO患者的轨道组织和血清中的GSDMD水平.
评估血清GSDMD和GO的临床活性评分 (CAS) 之间的相关性.
从GO患者培养的轨道纤维细胞 (OFs) 中阐明GSDMD介导的烧灭机制.

主要方法:

从GO患者的血清和轨道结合组织中定量GSDMD.
用TNF-α和IFN-γ治疗的培养GO患者的OFs,分析GSDMD表达和热的标志物.
利用siRNA在OF中创建GSDMD淘汰系统,用于功能研究.
测量IL-1β和IL-18的产量,作为对GSDMD操纵的反应.

主要成果:

血清GSDMD水平升高与GO患者的CAS正相关.
在GO轨道组织中发现了增加的GSDMD和N端GSDMD (NT-GSDMD) 表达.
实验室模型显示,在炎症刺激时,GSDMD和火灭菌因子增加.
通过NF-κB/AIM-2/caspase-1通路进行GSDMD介导的烧会导致GO炎症.
抑制GSDMD抑制了促炎性细胞因子的产生.

结论:

在GO中,GSDMD受到上调监管,并与疾病活动有关.
通过NF-κB/AIM-2/caspase-1通路,GSDMD介导的热致病导致GO的发生.
GSDMD代表了GO炎症的潜在生物标志物和治疗标.

关键词:

GSDMDMD的总体发展目标是什么格雷夫斯的轨道病 (GO)炎症炎症是一种炎症.轨道纤维细胞细胞.热性灭症 (Pyroptosis) 是一种

更多相关视频

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

相关概念视频

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...