相关实验视频
Updated: Jul 3, 2025
09:23
Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
13.8K
通过对工程RGI/BAK1的拉巴素诱导的二分化和BIR3仿真体来控制侧向根的形成
Byeong Wook Jeon1,2, Na Young Kang1,2, Won-Yong Park3
1Department of Bioenergy Science and Technology, Chonnam National University, Gwangju, Korea.
Physiologia plantarum
|February 11, 2024
概括
富含白的重复受体激酶 (LRR-RKs) 调节植物生长和免疫力. 这项研究表明,RGI受体与BAK1核受体的构成性激活抑制了横向根发育和初级根生长.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物信号通道的信号通道.
- 受体激酶的信号传递.
背景情况:
- 富含白的重复受体激酶 (LRR-RKs) 对于植物生长,发育和免疫力至关重要,可感知细胞外信号.
- 激活通常涉及连接体诱导的相互作用与SOMATIC胚胎发生受体KINASE (SERK) 核心受体.
- 根系生长因子1 (RGF1) 无敏性 (RGI) LRR-RLK受体调节主根活动并抑制侧根 (LR) 发育.
研究的目的:
- 探索RGI1,RGI4和RGI5的功能的增益,使用拉巴胺素诱导的二分化 (RiD) 和BAK1-交互的受体类似KINASE3 (BIR3) 仿真方法.
- 研究RGI-SERK核受体相互作用在植物发育中的作用.
主要方法:
- 采用拉巴胺素诱导的二分化 (RiD) 来诱导RGI细胞结核激酶域 (CKD) 与BAK1核受体之间的关联.
- 利用BIR3仿真构造来构成性地激活RGI受体.
- 分析了对转基因阿拉比多普西斯的横向根形成,初级根生长和根重力的影响.
主要成果:
- 拉巴胺素治疗诱导了RGI-BAK1协会,激活了MPK3和MPK6,并显著抑制了LR的形成.
- 表达RGI1CKD-BIR3仿真体的转基因植物在接受雌二醇治疗后显示了减少LR密度和减少根重力化.
- 使用BAK1对RGI的构成性激活抑制了初级根生长,类似于高植物激素度.
结论:
- 拉帕素或BIR3仿真体诱导的RGI CKDs和BAK1之间的相互作用有效地控制横向根部发育.
- RGI和SERK核受体之间的组合相互作用对于抑制根重力变是很重要的.
- 构成性RGI激活影响了关键的植物生长过程,为信号通路提供了洞察力.
相关概念视频
01:22PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
01:24Small GTPases - Ras and Rho
4.0K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
4.0K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K