光色控制的水溶液的pH调整,使用工程蛋白质体
Daniel Harder1,2, Noah Ritzmann2,3, Zöhre Ucurum1
1Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, 3012, Switzerland.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|February 11, 2024
概括
这项研究引入了一种新的囊泡系统,它使用不同的光颜色来控制微升尺度上的pH值变化. 这种光驱动的pH调整为合成生物学和微流体学中的应用提供了精确的控制.
科学领域:
- 合成生物学 合成生物学
- 微流体学 微流体学
- 生物化学 生物化学
背景情况:
- 在微升尺度上精确控制pH值对于各种研究,医疗和工业应用至关重要.
- 微尺度pH控制的现有方法在分辨率和灵活性方面可能受到限制.
研究的目的:
- 开发一种可控制光的囊泡系统,以在微升尺度上精确调节pH值.
- 为了证明系统在控制pH敏感过程中的能力.
主要方法:
- 工程化蛋白质体,包含在脂质膜中以相反的方向导向的细菌和蛋白质 (Med12).
- 利用不同的光颜色来驱动质子并诱导pH值变化.
- 实现了计算机控制的反循环,用于自动维护pH值.
主要成果:
- 在周围溶液中实现光引起的pH值变化.
- 显示了超过两个单位的pH控制范围,具有很好的时间和pH分辨率.
- 通过使用浅色成功控制了pH敏感酶反应的进展.
结论:
- 工程蛋白质体提供了一种新的方法,用于在微升尺度上以光控制调整pH值.
- 这项技术为控制合成生物学和其他领域的微尺度过程提供了新的可能性.
相关概念视频
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
pH Regulation in Cells
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Bioreactor Controls-I
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...


