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Updated: Jul 3, 2025

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从微滴中从不稳定的酶中获得基于吸收的单分子动态的快速获取

Yuhao Zheng1, Jinghu Chen1, Xinyi Wang1

  • 1Key Laboratory of Cardiovascular & Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China.

Small (Weinheim an der Bergstrasse, Germany)
|February 11, 2024
PubMed
概括

一种新的高通量成像方法使单分子催化物的无标签研究成为可能. 这种技术揭示了酶活性分布,并阐明了像马过氧化酶 (HRP) 等酶的逐渐变性机制.

关键词:
吸收光谱法是一种吸收光谱法.高吞吐量,具有高吞吐量.这些微粒是微滴.单个分子催化剂的催化.不稳定的酶是不稳定的.

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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