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温度适应对两个北极鱼类上层热耐受性的影响
Carolyn R Waterbury1, Trent M Sutton1, Amanda L Kelley2
1Department of Fisheries, University of Alaska Fairbanks, Fairbanks AK 99705, USA.
Conservation physiology
|February 12, 2024
概括
北极鱼类,大白鱼和沙夫兰鱼,在适应更温暖的水域时,显示出更高的耐热性和热冲击蛋白表达. 这种生理可塑性是它们在水温上升的情况下生存的关键.
科学领域:
- 水生生态学 水生生态学
- 生理生态生态学生理生态学
- 气候变化生物学
背景情况:
- 由于气候变化,北极的波福特海正在变暖.
- 大白鱼 (Coregonus nasus) 和沙弗朗鱼 (Eleginus gracilis) 是北极重要的生态鱼类.
- 了解它们的热耐受性对于预测生态系统对变暖的反应至关重要.
研究的目的:
- 为了确定5°C和15°C的适应如何影响大白鱼和沙夫兰鱼的临界热最大值 (CTmax).
- 评估适应温度对各种组织 (大脑,肌肉,肝脏) 中热冲击蛋白70 (HSP70) 表达的影响.
- 为了研究热耐受性和HSP70反应的特定物种和特定组织的差异.
主要方法:
- 鱼类适应5°C和15°C的温度.
- 使用标准化热升降速率 (3.4°C·h-1) 测量了临界热最大值 (CTmax).
- 在大脑,肌肉和肝脏组织中量化了HSP70蛋白和mRNA表达水平.
主要成果:
- 与适应5°C (分别为23.7°C和23.2°C) 的鱼相比,适应15°C的鱼具有显著更高的CTmax值 (27.3°C为大白鱼,25.9°C为沙夫兰鱼).
- 宽白鱼的CTmax和HSP70蛋白水平高于沙夫兰鱼,特别是在15°C的适应条件下.
- HSP70的表达 (蛋白质和mRNA) 根据组织而异,大脑的度最高,肌肉的度最低.
结论:
- 宽白鱼和蓝三文鱼都表现出生理可塑性,以应对升高的适应温度.
- 温暖气候的鱼类中CTmax和HSP70表达的增加表明它们对北极水温上升的适应能力.
- 这些发现对于了解北极鱼群对持续气候变化的抵御能力至关重要.
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