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Updated: Jul 3, 2025

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

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现代方法改善相位对比电子显微镜的现代方法.

Jeremy J Axelrod1,2, Jessie T Zhang1, Petar N Petrov1,2

  • 1Department of Physics, University of California Berkeley, Berkeley, CA 94720, USA.

ArXiv
|February 12, 2024
PubMed
概括

阶段板通过移动电子束相位来增强冷电子显微镜 (cryo-EM) 的对比度. 本综述涵盖了基于激光的方法和扫描传输电子显微镜 (STEM) 的高分辨率成像替代方案.

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Phase-Contrast Microscopes
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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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