全基因组转录组分析揭示了恐龙鞭毛动物中长非编码RNA的多样性和功能
Yibi Chen1, Katherine E Dougan1, Quan Nguyen2
1The University of Queensland, School of Chemistry and Molecular Biosciences, Australian Centre for Ecogenomics, Brisbane, QLD 4072, Australia.
NAR genomics and bioinformatics
|February 12, 2024
概括
研究人员在恐龙体中发现了数千种长非编码RNA (lncRNA),揭示了它们的多样性和在基因调节中的潜在作用,特别是在这些重要海洋微生物的热应激反应期间.
科学领域:
- 海洋生物学 海洋生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 丁诺鞭状动物是具有独特基因组特征的关键植物浮游生物.
- 长非编码RNAs (lncRNAs) 是真核生物中重要的基因调节剂,但在恐龙类动物中仍然基本未被研究.
研究的目的:
- 在三个具有生态意义的恐龙鞭动物物种中识别和描述IncRNAs.
- 研究 lncRNAs 的潜在功能,包括它们在应激反应中的作用.
主要方法:
- 分析来自 *Cladocopium proliferum*, *Durusdinium trenchii* 和 *Prorocentrum cordatum* 的高质量的基因组和转录组数据.
- 基于序列相似性,保存动机和预测功能的多基化lncRNAs的识别和分类.
- 在热应激下进行差异表达分析,并调查lncRNA基因共表达模式和目标预测.
主要成果:
- 鉴定了48,039个多基化lncRNAs,其中78.6%显示独特的序列.
- 与蛋白质编码序列相比,lncRNAs表现出较少的内子和较低的G + C含量.
- 3,708个lncRNAs (7.7%) 在热应激下被差异地表达,预计在基因调节和与蛋白质编码基因的共同表达中发挥作用.
结论:
- 这项研究提供了第一个关于恐龙鞭毛动物中lncRNAs的全面目录.
- lncRNAs在恐龙生物中扮演着不同的角色,并参与热应激反应.
- lncRNAs代表了一种新的调节层在恐龙体基因表达,特别是在环境压力下.
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