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研究欧鱼尼罗鱼 (Oreochromis niloticus) 中的透反应和myo-inositol氧酶的功能

Fan Zhang¹, Qiuran Yu¹, Yuxing Huang¹

¹Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, People's Republic of China.

American journal of physiology. Cell physiology

|February 12, 2024

概括

肌化醇氧酶 (MIOX) 对于尼罗河鱼的透调节和盐分耐受性至关重要. 它的基因表达在压力下增加,影响肌肉中毒醇水平,葡萄糖代谢和抗氧化剂防御.

科学领域:

水生动物生理学水生动物生理学
奥斯摩调节和应激反应
酶在新陈代谢中的功能

背景情况:

肌化醇氧化酶 (MIOX) 是肌化醇代谢中的关键酶,影响水生动物的肌化醇水平.
了解MIOX的作用对于尼罗河鱼适应不同度环境至关重要.

研究的目的:

为了研究myo-inositol氧化酶 (miox) 在尼罗河鱼的透调节中的功能.
确定myox对盐分耐受性,myo-inositol积累,葡萄糖代谢和氧化应激的影响.

主要方法:

在超的压力下对各种尼罗河鱼组织中myox基因表达的分析.
测量myox基因转录起始部位活动和对透/葡萄糖刺激的反应.
评估盐分耐受性,myo-inositol水平,葡萄糖代谢酶表达和氧化应激标志物在myox基因被淘汰后.

主要成果:

和肝脏的MIOX基因表达在和肝脏的表达最高,在超性压力下显著上调.
特定的促进器区域 (-1,640/-1,619和-620/-599) 被确定为对透和葡萄糖刺激的响应.
米奥克斯基因倒置降低了盐分耐受性,改变了myo-inositol积累,降低了葡萄糖代谢酶表达,降低了抗氧化剂性能.

结论:

MIOX在尼罗河鱼的透调节中起着至关重要的作用,并增强了耐盐度.
MIOX 影响肌肉内醇的积累,葡萄糖代谢和抗氧化能力,这对于适应高透性条件至关重要.

关键词:

尼罗河鱼是尼罗河鱼.- 伊诺西托尔氧化酶奥斯摩斯调节的调节.奥斯莫斯反应元素的奥斯莫斯反应元素.

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