在Trichoderma harzianum中诱导基酶:一种固态发酵方法,使用废物和小麦/商业基来合成基托醇糖
Cynthia Lizbeth López-García1, Guadalupe Guerra-Sánchez1, Fortunata Santoyo-Tepole2
1Departamento de Microbiología, Laboratorio de Bioquímica y Biotecnología de Hongos, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Ciudad de México, México.
Preparative biochemistry & biotechnology
|February 12, 2024
概括
固态发酵 (SSF) 在Trichoderma harzianum中有效诱导酶,使用废物和小麦. 小麦/花素产生了更高的酶活性和醇糖 (COS) 产量.
科学领域:
- 生物技术是生物技术.
- 酶技术 酶技术是一种
- 微生物发酵 微生物发酵
背景情况:
- 基因酶对于降解甲类动物和真菌中发现的生物聚合物基因来说至关重要.
- 固态发酵 (SSF) 为浸泡发酵提供了一个环保的替代方案,减少了用水量.
- 特里科德玛·哈尔齐安 (Trichoderma harzianum) 是一种众所周知的真菌,具有产生酶的潜力.
研究的目的:
- 通过使用固态发酵 (SSF) 来评估Trichoderma harzianum中的奇丁酶诱导.
- 为了比较废物基与商业基的疗效,用小麦作为酶生产的基质.
- 为了研究由诱导的酶产生的基托醇糖 (COS) 的产生和特征.
主要方法:
- 废弃物和小麦的预处理和表征,用于SSF.
- 在SSF条件下的Trichoderma harzianum种植,使用不同的基因基质.
- 用光谱光度和显微镜分析来评估真菌生长和酶活性.
- 由此产生的基托醇糖 (COS) 混合物的表征.
主要成果:
- 与废物素 (41.8 U/mg蛋白质) 相比,小麦/商业基因基质的基因酶活性显著更高 (76.3 U/mg蛋白质).
- 来自小麦/商业基的COS混合物显示了较高的降解糖度 (87.85毫米),而不是废基 (14.87毫米).
- 来自小麦/商业基的COS概况更为多样化,从单体到六体,而废弃基主要产生单体.
结论:
- 固态发酵是Trichoderma harzianum中提丁酶诱导和COS产生的一种有效方法.
- 小麦和商业化的组合是最大限度地提高酶活性和COS产量的优质基质.
- 该研究支持副产品的价值化和可持续生产有价值的化合物,如COS在各种行业的潜在应用.
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