机基酸盐排毒和乙烯基胆化酶重新激活由地质化伊米达酸框架引发的结构降解
Javier D Martin-Romera1, Emilio Borrego-Marin1, Pedro J Jabalera-Ortiz1
1Departamento de Química Inorgánica, Universidad de Granada, Av. Fuentenueva S/N, Granada 18071, Spain.
ACS applied materials & interfaces
|February 12, 2024
概括
二氧化伊米达酸框架 (ZIFs) 通过降解神经毒剂和恢复乙胆化酶 (AChE) 活性作为有机 (OP) 中毒的解药. ZIF-8纳米颗粒显示高效,没有神经毒性.
科学领域:
- 材料科学 材料科学 材料科学
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 有机酸盐 (OP) 毒性来自抑制乙胆酶 (AChE),这对神经传递至关重要.
- 开发对OP中毒的有效解药仍然是一个重大挑战.
研究的目的:
- 为了研究二氧化伊米达酸框架 (ZIFs) 作为对抗OP中毒的抗药物的潜力.
- 评估不同ZIFs在降解OP神经毒剂模型和重新激活ACHE方面的有效性.
主要方法:
- 合成和表征了各种ZIF与不同的imidazolate链接器.
- 测试的ZIF与G型神经毒剂模型化合物 (DIFP,二甲基酸盐) 相比.
- 评估了ZIF结构降解,OP水解和ACHE反应动力学.
- 使用神经母细胞瘤细胞培养物进行了体外神经毒性测定.
主要成果:
- ZIF中的Zn-L协调键对OP化合物敏感,导致P-X键的水解.
- ZIF 经历结构性降解,释放 imidazolate 链接剂和促进 OP 排毒的离子.
- Zn(2-methylimidazolate) 2 (sod ZIF-8) 纳米颗粒 (20 nm) 显示出最佳性能,DIFP降解半衰期为2.6分钟,AChE在1小时内完全恢复.
- 20nm sod ZIF-8纳米颗粒在细胞活力测试中没有表现出可观察到的神经毒性.
结论:
- ZIFs,特别是20nm sod ZIF-8纳米颗粒,显示为有机中毒的有效和安全抗剂的承诺.
- ZIFs的有效性受到链接核友性,框架拓和粒子大小的影响.
- 对基于ZIF的排毒剂进行进一步的研究是有必要的.
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