培养基底的刚性影响人体髓细胞质的收缩性依赖的增殖和核膜纹
Jo Nguyen1,2, Lu Wang2, Wen Lei2
1Institute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3E2, Canada.
Journal of cell science
|February 12, 2024
概括
肌肉干细胞的行为受到其微环境的影响. 纤维蛋白和基质刚度会影响细胞收缩和增殖,较软的基质会促进与细胞分裂相关的核纹.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 肌肉干细胞 (MSC) 再生对于组织修复至关重要.
- 微环境线索,如细胞外矩阵 (ECM) 连接体和基质刚度,显著影响细胞行为.
- 这些线索在衰老和疾病中的失调会损害肌肉再生.
研究的目的:
- 调查ECM连接体和基质刚度如何影响人类髓细胞收缩性和增殖.
- 根据基质刚度,发现细胞代谢,细胞骨和核部件的区别.
- 探索核形态,收缩性和扩散之间的关系.
主要方法:
- 主要的人类肌细胞培养在具有不同刚度和ECM连接体 (纤维素,拉米林,原) 的基质上.
- 细胞收缩性和增殖被测量在16小时内.
- 进行全蛋白质组分析以确定代谢和结构差异.
- 核形态 (纹) 和Ki67表达被分析和相关联.
主要成果:
- 与拉米林和原相比,绑定的纤维内素在肌细胞中诱导了更强的硬度依赖反应.
- 蛋白质组分析显示了软基质和硬基质之间的细胞代谢和细胞骨/核调节的差异.
- 较软的基板增加了神经细胞的核纹,这预测了Ki67的表达.
- 药理学上对收缩性的操纵影响了核纹和Ki67表达.
结论:
- ECM配体调节人体肌细胞硬度依赖的收缩性反应.
- 基质刚度会影响肌细胞质代谢,细胞骨组织和核结构.
- 核纹与肌细胞增殖有关,并且可以通过收缩性来调节,这表明潜在的细胞再生机制.
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