建立一个质量保证和质量控制的最佳实践框架,用于非目标代谢学
Jonathan D Mosley1, Tracey B Schock2, Chris W Beecher3
1Center for Environmental Measurement and Modeling, Environmental Protection Agency, Athens, GA, 30605, USA. mosley.jonathan@epa.gov.
Metabolomics : Official journal of the Metabolomic Society
|February 12, 2024
概括
建立质量保证 (QA) 和质量控制 (QC) 的最佳实践在非目标代谢学中对于可靠的数据至关重要. 代谢质量保证和质量控制联盟 (mQACC) 正在为协调的质量保证/质量控制协议制定路线图.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 质量保证 (QA) 和质量控制 (QC) 对于确保数据完整性在非目标代谢学中至关重要.
- 代谢质量保证和质量控制联盟 (mQACC) 和其最佳实践工作组 (WG) 致力于推进QA/QC实践.
- 协调和传播最佳实践是加强代谢学领域的关键.
研究的目的:
- 制定一个战略路线图,以实施质量保证/质量控制在非目标代谢学中的最佳实践.
- 通过标准化协议指导从业者,促进现场进展.
- 促进基本质量保证/质量控制活动的广泛采用,特别是液态染色体质谱 (LC-MS).
主要方法:
- 通过研讨会,论坛和调查进行社区参与,以收集有关质量保证/质量控制实践的意见.
- 根据工作组讨论和社区反,确定和优先考虑七个主要的质量控制阶段.
- 开发一个框架来识别和编目质量保证/质量控制的最佳实践.
主要成果:
- 通过各种互动活动,在优先级的质量控制阶段收集了社区的意见.
- 正在建立一个结构化的方法来识别和定义质量保证/质量控制的最佳实践.
- 这个过程涉及到每一个质量控制阶段内的多项活动的详细讨论和反.
结论:
- mQACC最佳实践工作组正在为非目标代谢学QA/QC创建一个"生活指南"文件.
- 这个活着的文件将随着现场发展而发展,确保当前和相关的最佳实践.
- 该倡议旨在提高全球非向代谢学研究的可靠性和可重复性.
相关概念视频
01:05Quality Control
Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
01:19Quality Assurance
Quality assurance is the overarching term used to describe the activities employed to ensure the proper performance of a system. These activities can be classified into three categories: quality control, quality assessment, and internal corrective measures. Typically, these activities work cyclically: quality control is performed before and during the analysis, while quality assessment occurs during and after the investigation. Internal corrective measures are implemented based on the findings...
01:15Data Validation
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
Key parameters for method validation include:
01:26Good Manufacturing Practices
Good Manufacturing Practices (GMP) constitute a foundational set of guidelines that ensure the production of safe, consistent, and high-quality products, particularly in industries such as pharmaceuticals, biotechnology, and food processing. These protocols encompass all aspects of production, from the sourcing of raw materials to the final distribution of the finished product.A core pillar of GMP is stringent hygiene and sanitation across all production environments. This includes routine...
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:28Bioreactor Controls-I
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...