在Situ中,转胺酶交叉链接改善了生物医学应用的自组合的机械性能
Maria Gessica Ciulla1, Amanda Marchini1,2, Jacopo Gazzola3
1Institute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies, IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
ACS applied bio materials
|February 12, 2024
概括
研究人员使用转胺酶 (TGase) 酶交叉链接增强了自我组装的 (SAP) 水凝. 这提高了硬度和弹性,而不会损害细胞活力,为体内应用铺平了道路.
科学领域:
- 生物材料科学 生物材料科学
- 纳米医学是一种纳米医学.
- 组织工程是组织工程.
背景情况:
- 三维 (3D) 生物材料对于组织修复至关重要,自组装 (SAP) 成为模仿细胞外基质 (ECM) 的有希望的生物相容支架.
- 软SAP水凝需要增强的刚性和弹性,以有效地恢复生理功能.
- 转胺酶 (TGase) 2型可以形成异类键,为水凝交叉链接提供了一个潜在的策略.
研究的目的:
- 使用TGase开发一种酶策略,以提高功能化SAP的刚性和弹性.
- 评估TGase交联对SAP生物材料的机械特性和细胞反应的影响.
主要方法:
- 功能化的SAP序列的合成和表征.
- 使用TGase 2型的SAPs的酶交叉链接.
- 病理学实验,原子力显微镜 (AFM),硫黄-T结合测定和ATR-FTIR光谱测试以评估交联和机械性质.
- 使用人类神经干细胞 (hNSC) 进行体外试验,以评估细胞活力和分化.
主要成果:
- TGase交叉连接显著提高了SAP的存储模块.
- 在不损害最大应力-在-失败的情况下,实现了增强的刚性.
- TGase交叉链接并没有阻碍种子hNSCs的生存或分化.
结论:
- 与TGase进行酶交联是一种有效的策略,可以增强SAP水凝的机械性能.
- 这种方法保持了细胞活力和分化,这表明了 in vivo 安全的 in situ 交叉链接应用的潜力.
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