相关实验视频

Updated: Jul 3, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

考古以太脂质改善了与mRNA脂质纳米颗粒的内化和转染

Viktor Laurin Sedlmayr¹, Silvia Schobesberger², Sarah Spitz²

¹Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Straße 1a, Vienna 1060, Austria.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

|February 12, 2024

概括

考古以太脂质 (AELs) 显示出提供mRNA的前景. 这些脂质显著增强了细胞中的mRNA表达,为下一代传递系统提供了新的途径.

科学领域:

生物化学生物化学
分子生物学分子生物学
药物输送系统药物输送系统

背景情况:

考古以太脂质 (AEL) 已知具有免疫记忆的安全pDNA传递.
目前的mRNA传递系统,如脂质纳米粒子 (LNP),正在迅速发展.
在mRNA输送中AELs的潜力仍然未被探索.

研究的目的:

为了研究古老乙烯脂质 (AELs) 作为mRNA LNP的新型辅助剂.
为了评估来自Sulfolobus acidocaldarius的AELs在mRNA输送中的有效性.
为了比较基于AEL的LNP与已建立的交付配方.

主要方法:

使用了来自Sulfolobus acidocaldarius的原始脂质提取物和纯化的卡尔达凯奥尔.
配制的mRNA LNP包含不同摩尔比的AEL.
使用人类骨肌细胞 (HSMM) 和Caco-2,C2C12细胞系进行了体外转染实验.

主要成果:

与参考配方相比,含有AEL的LNP增加了多达10倍的颗粒吸收和mRNA表达.
效率取决于AEL在LNP中的摩尔份额.
观察到增强的内分体逃逸,这表明AELs具有融合性和解热性特性.

结论:

考古以太脂质显示出作为mRNA输送系统的有价值组件的巨大潜力.
AELs可以显著改善mRNA表达水平和细胞吸收.
这项研究为未来的体内研究和先进的mRNA治疗方法的开发奠定了基础.

关键词:

考古物体是指古物体.细胞内体逃脱是什么?脂质纳米颗粒的使用方法乙烯脂质是四乙烯的脂质.疫苗疫苗疫苗疫苗它们是mRNARNA.

更多相关视频

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

相关概念视频

Assembly of the Lipid Bilayer in the ER

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...

Asymmetric Lipid Bilayer

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...