通过染色学与高分辨率质谱学相结合的化的量化 - - 部分B:液态染色学分离的影响
Cherine Amoura1, Frédéric Larvor1, Philippe Marchand1
1Oniris, INRAE, LABERCA, F-44300, Nantes, France.
Chemosphere
|February 12, 2024
概括
由于复杂的混合物,分析化 (CPs) 是具有挑战性的. 这项研究优化了液体染色学-电子喷雾电离-高分辨率质谱学 (LC-ESI-HRMS) 以准确地确定食品中的CP.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 食品安全 食品安全
背景情况:
- 由于复杂的多基 (PCA) 混合物,化 (CPs) 存在分析挑战.
- 同源共和电离干扰使准确的CP分析变得复杂.
- 对短链CP的监管重点需要改善食品矩阵的分析方法.
研究的目的:
- 使用LC-ESI-HRMS调查染色分离对PCA同类组模式的影响.
- 优化LC-ESI-HRMS条件,以准确确定食品样本中的CP.
- 评估不同的移动阶段和LC列,以改进CP分析.
主要方法:
- 三个移动相 (MeOH/H2O,ACN/H2O) 和六个LC柱的评估.
- 优化LC-ESI-HRMS合用于CP分离和检测.
- 从使用优化条件的实验室间研究中,在食品样本中量化PCA.
主要成果:
- MeOH/H2O移动相更好地揭示了高化PCA,而ACN/H2O产生了较少的离子物种.
- 超碳水化合物列显示出明显的PCA模式,这是由于强烈的保留.
- C30列有效地分离了短链CP,性能优于传统的C18列.
- 使用C30列和MeOH/H2O的优化LC-ESI-HRMS实现了食品样本中PCAs的准确和精确量化.
结论:
- LC-ESI-HRMS,特别是使用C30列和MeOH/H2O,显著改善了化抛的分析.
- 优化的方法为食品中的CP确定提供了令人满意的准确性和精度,满足了对短链CP的监管需求.
- 这一进步有助于在食品安全评估中对CP进行可靠的监测.
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