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定量蛋白质和新陈代谢分析揭示了不同细胞的不同度调节机制,这些细胞应对不同的超度应激

Jingyu Pan¹, Minxu Wang¹, Jiahua Zhu¹

¹Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai 200241, China.

Journal of proteomics

|February 12, 2024

概括

欧鱼细胞利用不同的策略来生存透应激. 常规的压力涉及合成氧化物,而极端的压力会通过特定的信号通路触发细胞亡和细胞循环停止.

科学领域:

* 分子生物学和细胞生物学
* 鱼类生理学和透调节
* 蛋白质学和代谢学

背景情况:

* 了解鱼类透调节对于水产养殖和环境研究至关重要.
*将透应激传感与细胞反应联系起来的分子机制仍然不太清楚.
*以前的研究集中在离子载体上,忽视了信号通路和代谢调整.

研究的目的:

* 为了研究不同高度应激条件下的欧鱼细胞调节机制.
* 探索信号通路和代谢变化在度调节中的作用.
* 为了确定关键因素,关键的细胞存活在透挑战期间.

主要方法:

*暴露Oreochromis mossambicus大脑 (OmB) 细胞以控制,定期和极端的高位压力.
* 暴露12小时后的蛋白质学和代谢学分析.
*对奥米克数据进行综合分析,以了解分子反应.

主要成果:

* 这两种压力类型都减少了细胞生长和改变了形态.
* 定期的高透性压力诱导了相容的有机透酸盐合成.
* 极度高位应激激活了细胞亡和细胞循环停止,涉及PI3K-Akt,p53,HIF-1,FoxO,JAK-STAT和Hippo通路.

结论:

* 欧鱼采用基于压力强度的独特分子调节策略来调节.
*信号通路在调解细胞对透挑战的反应中起着关键作用.
* 这项研究提供了关于鱼细胞透调节的复杂分子相互作用的见解.

关键词:

超的压力是超的压力欧米克斯的综合性分析.代谢学代谢学代谢学透调节机制的机制蛋白质组学是指蛋白质组学.

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